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Quantification of 11C-PIB kinetics in mouse brain using metabolite-corrected arterial input function
https://repo.qst.go.jp/records/62644
https://repo.qst.go.jp/records/626448a0ae150-d096-41ce-a130-4911a5a25124
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-07-24 | |||||
| タイトル | ||||||
| タイトル | Quantification of 11C-PIB kinetics in mouse brain using metabolite-corrected arterial input function | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Seki, Chie
× Seki, Chie× et.al× 関 千江 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: Recently, small animal PET studies have become of interest because various disease models have been developed by genetic manipulations primarily in mice. Advantages of PET study include in vivo evaluation of tracer kinetics that reflects physiological parameters quantitatively. Generally tracer kinetic analysis requires metabolite-corrected arterial input function obtained as plasma time-activity curve (pTAC). In this study, we performed pTAC measurement during the dynamic PET scan to test the capability of quantitative PET studies in mice using an amyloid tracer, 11C-PIB. \nMethods: Six male C57BL/6J mice (age, 9-10 weeks; body weight, 23-26g) were anesthetized with isoflurane throughout the experiment. The femoral artery was canulated to withdraw arterial blood, and mice were positioned in the micro-PET Focus 220 scanner. Simultaneously with the intravenous 11C-PIB (0.4-1.8 mCi, 0.2 ml) injection, 90-minute PET data acquisition and arterial blood sampling were started. Totally 12-14 blood samples were taken during the scan. The amount of each plasma sample was determined with micropipette and the radioactivity was measured with auto-gamma counter. A subset of plasma samples underwent HPLC analysis to determine unchanged 11C-PIB fraction. Tissue time-activity curves (tTACs) in the neocortex and cerebellum were obtained from dynamic PET data and total volume of distribution (VT) was estimated with one-tissue compartment model analysis (1TCM) and graphical analysis (GA) [1]. Furthermore, 2 mice were retested 4 weeks after the first experiment and reproducibility was assessed by variability: Variability (%) = 100x(test-retest)/{(test+retest)/2}. \nResults: Fig. 1 shows the time course of mean unchanged 11C-PIB fraction in plasma. Examples of pTAC and cerebeller tTAC from a test/retest study are shown in Figs. 2 and 3. VTs in the cerebellum and neocortex estimated with 1TCM were 1.1+/-0.2 and 0.82+/-0.22, respectively, and those estimated with GA were 1.4+/-0.4 and 1.1+/-0.4, respectively (ml/cm3; mean+/-sd). They were approximately 1/3 of those in humans reported previously [2]. Variability of estimated VT with 1TCM was ranging from 10% (cerebellum) to 27% (neocortex) and that with GA was from 5% (cecebellum) to 26% (neocortex). Conclusion: Our study has demonstrated the applicability of kinetic analysis using metabolite-corrected pTAC to PET measurements in small animals. The variability of estimated VT was mostly attributable to inaccurate mass measurement of small-volume plasma in the micro-liter range. With additional technical improvements, the present system would contribute to quantitative PET studies with diverse radiotracers, including those for which the reference region is not available. \n[1] Logan, J., et al. J Cereb Blood Flow Metab: 10, pp. 740-747, 1990. [2] Price, C.J., et al. J Cereb Blood Flow Metab: 25, pp. 1528-1547, 2005. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The Seventh International Symposium on Neuroreceptor Mapping of Living Brain | |||||
| 発表年月日 | ||||||
| 日付 | 2008-07-19 | |||||
| 日付タイプ | Issued | |||||
