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Comparative studies on DNA double strand breaks induced by sulforaphane and X-rays
https://repo.qst.go.jp/records/62582
https://repo.qst.go.jp/records/62582894135a2-fe1e-4729-8138-884e6d806e43
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-06-23 | |||||
| タイトル | ||||||
| タイトル | Comparative studies on DNA double strand breaks induced by sulforaphane and X-rays | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sekine, Emiko
× Sekine, Emiko× 関根 絵美子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Sulforaphane (l-isothiocyanate-4methylsulfinylbutane: SFN), an isothiocyanate, isolated from broccoli and other cruciferous vegetables is wellkown for cancer preventive agent. It was reported that SFN suppressed proliferation of cancer cells by cell cycle arrest and/or by inducing apoptosis by activating the caspase apoptosis pathway. After an accidental discovery of SFN inducing DNA strand breaks in our laboratory, we started to systematically study DNA double-strand breaks (DSBs) induced by this product, and the result was compared to that with X-rays. DSB is thought to be the most critical lesions for radiation induced damage and cells have severe biological consequences if this damage is not repaired or mis-repaired. HeLa cells were exposed to various concentrations of SFN and the cell growth was measured. The induction of DNA DSB was examined using constant field gel electrophoresis (CFGE) and gamma-H2AX assay. Our data revealed that cell growth was impaired in a SFN dose dependent manner. DNA DSBs were also induced in SFN dose and time dependent manners. The amount of DSBs induced by 20μM SFN for 48h, for example, correspond to that induced by 10 Gy X-rays. Moreover, our immuno-staining experiments indicated the appearance of Rad51 (homologous recombination repair (HRR) related protein) foci observed in a dose and time dependent manner along with the DNA DSB production. In contrast, there was no phosphorylation of DNA-PKcs (a critical non-homologous end joining (NHEJ) protein) observed in SFN exposed cells. In summary our data clearly demonstrate the induction of DNA DSBs by SFN, and these breaks seem to be predominantly repaired by the HRR pathway. These results give a possibility that SFN could be used as an effective anticancer agent. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Workshop on Radiation Damage to DNA | |||||
| 発表年月日 | ||||||
| 日付 | 2008-06-12 | |||||
| 日付タイプ | Issued | |||||
