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Classifying the variation in response to ionizing radiation in human cells by gene expression profiling

https://repo.qst.go.jp/records/61298
https://repo.qst.go.jp/records/61298
57cf86c7-31ee-4ec0-99e4-b6f3b76aa327
Item type 会議発表用資料 / Presentation(1)
公開日 2006-02-09
タイトル
タイトル Classifying the variation in response to ionizing radiation in human cells by gene expression profiling
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Ishikawa, Kenichi

× Ishikawa, Kenichi

WEKO 606757

Ishikawa, Kenichi

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Saegusa, Kumiko

× Saegusa, Kumiko

WEKO 606758

Saegusa, Kumiko

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Ootsuka, Yoshimi

× Ootsuka, Yoshimi

WEKO 606759

Ootsuka, Yoshimi

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Ishikawa, Atsuko

× Ishikawa, Atsuko

WEKO 606760

Ishikawa, Atsuko

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Yasuda, Kaori

× Yasuda, Kaori

WEKO 606761

Yasuda, Kaori

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Furuno, Aki

× Furuno, Aki

WEKO 606762

Furuno, Aki

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Kawai, Seiko

× Kawai, Seiko

WEKO 606763

Kawai, Seiko

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Kimura, Tomo

× Kimura, Tomo

WEKO 606764

Kimura, Tomo

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Michikawa, Yuichi

× Michikawa, Yuichi

WEKO 606765

Michikawa, Yuichi

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Harada, Yoshinobu

× Harada, Yoshinobu

WEKO 606766

Harada, Yoshinobu

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Iwakawa, Mayumi

× Iwakawa, Mayumi

WEKO 606767

Iwakawa, Mayumi

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Suzuki, Masao

× Suzuki, Masao

WEKO 606768

Suzuki, Masao

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Imai, Takashi

× Imai, Takashi

WEKO 606769

Imai, Takashi

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石川 顕一

× 石川 顕一

WEKO 606770

en 石川 顕一

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三枝 公美子

× 三枝 公美子

WEKO 606771

en 三枝 公美子

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荘司 好美

× 荘司 好美

WEKO 606772

en 荘司 好美

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石川 敦子

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WEKO 606773

en 石川 敦子

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安田 香央里

× 安田 香央里

WEKO 606774

en 安田 香央里

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古野 亜紀

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WEKO 606775

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川井 聖子

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WEKO 606776

en 川井 聖子

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菅 智

× 菅 智

WEKO 606777

en 菅 智

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道川 祐市

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WEKO 606778

en 道川 祐市

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原田 良信

× 原田 良信

WEKO 606779

en 原田 良信

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岩川 眞由美

× 岩川 眞由美

WEKO 606780

en 岩川 眞由美

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鈴木 雅雄

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WEKO 606781

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今井 高志

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WEKO 606782

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抄録
内容記述タイプ Abstract
内容記述 AIM To predict an unusual response to clinical radiations, identification of molecular markers and understanding the variation in radiosensitivity at molecular level are required. In this study, microarray assays were performed on the various cell lines to select the candidate genes as biomakers for radiosensitivity. METHODS Sixteen cell lines including two normal fibroblast cell strains and 13 cancer cell lines were cultured in Eagle's minimum essential medium (MEM) supplemented with 10% FBS. From survival curve of each cell line, D10 value, i.e. one of the parameters, was calculated. D10 value is the dose (Gy) required to reduce the surviving fraction to 0.1, and was defined as the phenotype of each cell line radiosusceptibility. Total RNA was purified from each cell line before irradiation, one or three hours after irradiation. Fluorescent complementary RNA was prepared by reverse transcription and "in vitro" RNA synthesis with Cy3- or Cy5- labeled nucleotides. Hybridization was performed with the custom-made oligonucleotide microarray containing 22,500 probes (Agilent Technologies, CA). After hybridization, slides were washed and scanned using a confocal laser scammer (Agilent Technologies, CA). Gene expression analysis was performed using RESOLVER (Rosetta Inpharmatics, WA) to search for genes whose levels of expression relate to the value of D10. RESULTS
Several gene sets were constructed as follows: genes induced in the radioresistant cells but not in the sensitive cells after irradiation or genes repressed in the radioresistant cells but not in the sensitive cells after irradiation. Expression ratio and measurement accuracy in the microarray experiments were considered for selection of the gene sets. When some gene sets were subjected to the two-dimensional cluster analysis, the cell lines whose D10 value was more than 6 Gy were clearly separated from the cell lines whose D10 values was relatively low, less than 4 Gy. The cell lines whose D10 value ranged between 4 and 6 Gy were also roughly ordered by the gene sets with the Classification tool of the Resolver. CONCLUSIONS Detection of the expressed genes selected by the microarray analysis is useful to predict radiosensitive or resistant cells that were clarified by D10 value.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 The 7th International Symposium on Predictive Oncology
発表年月日
日付 2004-02-10
日付タイプ Issued
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