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Comet assay, a possible screening assay to classify subgroups of individuals with different radiosensitivity using high throughput scanning system for multiple samples of human blood lymphocytes
https://repo.qst.go.jp/records/59930
https://repo.qst.go.jp/records/59930db580f72-3aa4-4229-b8d9-e39516299e88
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-09-02 | |||||
| タイトル | ||||||
| タイトル | Comet assay, a possible screening assay to classify subgroups of individuals with different radiosensitivity using high throughput scanning system for multiple samples of human blood lymphocytes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Iwakawa, Mayumi
× Iwakawa, Mayumi× Gotou, Miyako× Suga, Tomo× Noda, Syuuhei× Harada, Yoshinobu× Imai, Takashi× 岩川 眞由美× 中渡 美也子× 菅 智× 野田 秀平× 原田 良信× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This research was designed to identify the correlation between clinical radiosensitivity among breast cancer patients and in vitro radiosensitivity measured by alkaline comet assay in high throughput fashion. In 62 patients with breast cancer and age-matched 41 healthy volunteers, acute adverse effects on skin after radiotherapy were clinically scored according to RTOG grading system. Maximum score during 6 months after radiotherapy was grade 0 for 9 patients, grade 1 for 25 patients, grade 2 for 24 patients, and grade 3 for 4 patients. The parameters of alkaline comet assay were initial damage, which was mean tail moment (MTM) values in irradiated cells in vitro immediately after irradiation with 5 Gy, and % residual damage (RD) at 15 min after irradiation. Correlation between initial damage and skin reaction was found in breast cancer patients with grade 1 and grade 2, 3 (p=0.017). There was no correlation between RD and skin reaction (p=0.056), while large inter-individual variation of RD was revealed among breast cancer patients with grade 0 (17.04 +13.31 %), or grade 2, 3 (16.86 +11.09 %). By introducing a new analyzer, throughput of the comet assay data was highly improved. Our data suggests that the comet assay might be one of supportive assays to classify subpopulation of patients who have different radiosensitivity from normal responders with a fair-poor discriminating capacity of the test to identify the patients with higher risk of developing a severe acute reaction. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 12th International Congress of Radiation Research(ICRR) | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-22 | |||||
| 日付タイプ | Issued | |||||
