WEKO3
アイテム
DNA Fragmentation Induced by Protons and Fe-ions in Human Cells
https://repo.qst.go.jp/records/59354
https://repo.qst.go.jp/records/59354afd253da-8200-4129-86e4-801f3a962ab5
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-07-18 | |||||
| タイトル | ||||||
| タイトル | DNA Fragmentation Induced by Protons and Fe-ions in Human Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Antonelli, Francesca
× Antonelli, Francesca× Belli, Mauro× Cherubini, R× Dini, Valentina× Esposito, G× Furusawa, Yoshiya× Simone, Giustina× Sorrentino, Eugenio× Antonella, Tabocchini Maria× 古澤 佳也 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Protons and high energy heavy ions (HZE particles ) are the components of space radiation of major concern. Their biological consequences are expected to be related to DNA lesions. Since DNA double strand breaks (DSB) and other lesions produced by these charged particles occurs in a correlated manner along the particle track, the current working hypothesis is that spatial correlation of damage, that depends on the particle type and energy, affects its reparability. Several characteristic distances for damage correlation can be considered, related to various levels of chromatin organization. We have investigated, in AG1522 human fibroblasts, the DNA fragmentation patterns in the size range 23 kbp - 5.7 Mbp, characteristic of the loop structure of chromosomes, using calibrated Pulsed Field Gel electrophoresis (PFGE). We have compared various beams i.e., low -energy protons of 0.88 MeV (representative of stopping protons at the Bragg peak) and Fe ions of 1050 and 414 MeV/u, using g-rays as reference. Irradiations with protons were performed at the Laboratori Naz ionali di Legnaro (Padova, Italy), those with 1050 MeV/u Fe ions at the Brookhaven National Laboratory (USA) and those with 414 MeV/u Fe ions at the National Institute for Radiological Sciences (Chiba, Japan). DSB yields were evaluated from the number of DNA fragments induced in the size range studied, at doses between 40 and 200 Gy. The results until now obtained show the general trend of a linear, or almost linear, increase of DSB with the dose. 1050 MeV/u Fe ions are the most effective particles in terms of both unit dose and unit fluence. The frequency distributions of fragments induced by the charged particles are shifted towards smaller sizes with respect to that induced by comparable doses of g-rays. These distributions also suggest that at doses of the order of 100 Gy most of the fragments induced by protons have size > 23 kbp, while for Fe ions it is expected a significant amount of fragments smaller than that size, not revealed by the mentioned PFGE analysis. This is in agreement with recent experimental measurements of DNA fragments induced by 1050 MeV/u Fe ions in the size range 2 - 23 kbp. The occurrence of such small fragments (and even smaller) further increases the effectiveness for DSB induction of HZE particles compared to protons. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 34th COSPAR Scientific Assembly | |||||
| 発表年月日 | ||||||
| 日付 | 2002-10-19 | |||||
| 日付タイプ | Issued | |||||
