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Induction of the GADD45a gene by ionizing radiation in human myeloblastic leukemia ML-1 cells
https://repo.qst.go.jp/records/56930
https://repo.qst.go.jp/records/569307c5fe3da-3508-4154-a46d-b6fb216dbca7
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-12-27 | |||||
| タイトル | ||||||
| タイトル | Induction of the GADD45a gene by ionizing radiation in human myeloblastic leukemia ML-1 cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Daino, Kazuhiro
× Daino, Kazuhiro× Ichimura, Sachiko× Nenoi, Mitsuru× 臺野 和広× 沼田 幸子× 根井 充 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The growth arrest and DNA damage-inducible protein 45a (GADD45a) gene is a small acidic protein, inducible by various kinds of DNA-damaging stimuli including ionizing radiation and stress associated with growth arrest. It has been well documented that induction of the GADD45a gene after ionizing radiation exposure is regulated in a p53-depending manner. However, precise mechanism of induction of the GADD45a gene by ionizing radiation is not elucidated. In previous study, we showed that X-ray irradiation increased the transcription rate of the GADD45a gene much earlier than the maximum accumulation of stabilized p53 protein in human myeloblastic leukemia ML-1 cells. We hypothesized that some transcription factor(s) may cooperate with p53 in regulating the GADD45a gene early after the irradiation of ML-1 cells. This idea is supported by recent studies showing that the p53-dependent activation of several genes in human and mouse cells requires some additional transcription factors, such as Sp1, GKLF, Ets1, and IRF-1. To examine the possible involvement of cooperating factors in transcriptional regulation of the GADD45a gene by ionizing radiation, we comprehensively searched for the X-ray-inducible binding locus of the nuclear factor throughout the upstream region (-2244 bp/+89 bp) and the third intron (+1389 bp/ + 2488 bp) of the GADD45a gene by EMSA using 136 probes. The X-rayresponsive binding of nuclear factors was detected at eight loci. Oct, NF-kappaB, HNF, NF-AT, and KLF family transcription factors were identified by a competition assay. It is possible that some of these factors cooperate with p53 to mediate transcriptional regulation of the GADD45a gene after ionizing radiation exposure. | |||||
| 書誌情報 |
Recent Research Developments in Genes & Genomes 巻 1, p. 83-106, 発行日 2005 |
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| 出版者 | ||||||
| 出版者 | Transworld Research Network | |||||
