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Microbeams of heavy charged particles
https://repo.qst.go.jp/records/56833
https://repo.qst.go.jp/records/5683384100d42-12a9-44f9-8c01-440fcdc3d36e
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-06-02 | |||||
| タイトル | ||||||
| タイトル | Microbeams of heavy charged particles | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kobayashi, Yasuhiko
× Kobayashi, Yasuhiko× Funayama, Tomoo× Wada, Seiichi× Furusawa, Yoshiya× Aoki, Mizuho× Shao, Chunlin× Sakashita, Tetsuya× Matsumoto, Yoshitaka× Yokota, Yuichiro× 小林 泰彦× 舟山 知夫× 古澤 佳也× 青木 瑞穂× 坂下 哲哉× 松本 孔貴 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have established a single cell irradiation system, which allows selected cells to be individually hit with defined number of heavy charged particles, using a collimated heavy-ion microbeam apparatus at JAERI-Takasaki. This system has been developed to study radiobiological processes in hit cells and bystander cells exposed to low dose and low dose-rate high-LET radiations, in ways that cannot be achieved using conventional broad-field exposures. Individual cultured cells grown in special dishes were irradiated in the atmosphere with a single or defined numbers of 18.3 MeV/amu 12C, 13.0 MeV/amu 20Ne, and 11.5 MeV/amu 40Ar ions. Targeting and irradiation of the cells were performed automatically at the on-line microscope of the microbeam apparatus according to the positional data of the target cells obtained at the off-line microscope before irradiation. The actual number of particle tracks that pass through cell nuclei was detected with prompt etching of the bottom of the cell dish made of ion track detector TNF-1 (modified CR-39), with alkaline-ethanol solution at 37deg C for 15-30 minutes. Using this system, separately inoculated Chinese hamster ovary cells, confluent normal human fibroblasts, and single plant cells (tobacco protoplasts) have been irradiated. These are the first studies in which single-ion direct hit effect and the bystander effect have been investigated using a high-LET heavy particle microbeam | |||||
| 書誌情報 |
Biological Sciences in Space 巻 18, 号 4, p. 235-240, 発行日 2004-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0914-9201 | |||||
