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COX-2を指標とした炭素線誘発バイスタンダー応答に対する線量およびLET依存性の解析
https://repo.qst.go.jp/records/56142
https://repo.qst.go.jp/records/561422c34d9ba-7fc3-4761-9f21-170fcaf79cb0
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-02-12 | |||||
| タイトル | ||||||
| タイトル | COX-2を指標とした炭素線誘発バイスタンダー応答に対する線量およびLET依存性の解析 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
小林, 亜利紗
× 小林, 亜利紗× 大澤, 大輔× 古澤, 佳也× Nahathai, Dukaew× テングゥ, アーマッド テングゥ アブリザル ファリザル× Jun, Wang× 小西, 輝昭× Kobayashi, Alisa× Ohsawa, Daisuke× Furusawa, Yoshiya× Dukaew, Nahathai× Ahbrizal Farizal TENGKU AHMAD, Tengku× Konishi, Teruaki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In radiation cancer therapy, heavy ion will be penetrating the surface of the body to the targeted cancer region, which results various LETs of ions exposed in the patient’s body. Bystander effect of different LETs as well as radiation quality should be a taken into concern for radiation risks. In this study our aim is to evaluate LET dependence of bystander response focused on cyclooxygenase-2 (COX-2) as one of the mediator for bystander effect. For this fiscal year, we obtained survival curves for two LETs (13 keV /µm and 70 keV/μm) of 290 MeV/n carbon ion beam to confirm the induction of cell inactivation. Secondly, we measured the induction of COX-2 in both target and bystander cells by western blot analysis. As a result, COX-2 protein expression showed a gradual increase to 8 hrs post irradiation only with 70 keV/µm and not with 13 keV/µm. COX-2 in irradiated cells were 1.6 folds compared to the control. As for the bystander cells, we found that COX-2 increased only in the bystander cells co-cultured with the cells irradiated with LET 70 keV/µm, which was 1.4 folds compared to the controls. We have irradiated various doses with above two LETs and collected proteins. The COX-2 protein expression in both irradiated and bystander cells are currently under analysis, and the results will be reported in near future. |
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| 書誌情報 |
平成29年度放射線医学総合研究所 重粒子線がん治療装置等共同利用研究報告書 p. 77, 発行日 2018-09 |
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