WEKO3
アイテム
Evaluation system of DNA lesions caused by ion beam irradiation using the polymerase chain reaction
https://repo.qst.go.jp/records/56032
https://repo.qst.go.jp/records/56032722bcc34-4928-470b-a1eb-0fc901163381
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-04-14 | |||||
| タイトル | ||||||
| タイトル | Evaluation system of DNA lesions caused by ion beam irradiation using the polymerase chain reaction | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matuo, Y.
× Matuo, Y.× Izumi, Y.× 坂本, 綾子× 長谷純宏× K.Shimizu× 坂本 綾子× 長谷 純宏 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have been studying ion-beam induced mutations in budding yeast S288c (RAD+), as a model of the eukaryote cell. In this study, we tried to evaluate the amount of DNA lesion caused by high LET ion beams in the URA3 gene. The DNA sample was irradiated with carbon ions (LET: 107 keV/μm) and neon ions (LET: 335 keV/μm) with the dose of 1 - 100 Gy at TIARA. For comparison purpose, the DNA samples were also irradiated with helium ions (LET: 2.2 keV/μm) at HIMAC (National Institute of Radiological Sciences, Japan). Real-time PCR was employed to amplify a 210-bp region of the URA3 gene by using Real-Time PCR System. The ratio of replicable DNA decreased along with increasing dose in all three types of ion beams. This result suggests that our method can be used to evaluate the biological effects caused by various types of radiation with different LET. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2015 p. 134, 発行日 2017-03 |
|||||
