WEKO3
アイテム
Interphase Death Was Related to Growth Inhibition after Gamma-ray and Carbon-ion Irradiation in Human Neural Stem Cells but not in Glioblastoma Cells
https://repo.qst.go.jp/records/56025
https://repo.qst.go.jp/records/56025b188c25c-4a75-4286-bfae-cc613424d1fd
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-03-30 | |||||
| タイトル | ||||||
| タイトル | Interphase Death Was Related to Growth Inhibition after Gamma-ray and Carbon-ion Irradiation in Human Neural Stem Cells but not in Glioblastoma Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
横田, 裕一郎
× 横田, 裕一郎× 和田, 優× 舟山, 知夫× 横田 裕一郎× 舟山 知夫 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Neural stem cells (NSCs) have been found in the brain and spinal cord areas of various species including adult humans. They are self-renewing multipotent cells that primarily differentiate into neurons, astrocytes and oligodendrocytes in the process of neurogenesis. Because it is known that NSCs are severely damaged and neurogenesis is inhibited after X-ray and gamma-ray irradiation, radiation protection of NSCs during cranial irradiation of the patients with brain, neck and head tumors is needed to keep their quality of life. Increasing numbers of the patients have selected carbon-ion and proton radiotherapies because of their well-known better dose distribution. However, there is a limited piece of knowledge on biological responses of NSCs after irradiation of ion beams. The final purpose of this study is to elucidate the effects of ion-beam irradiation to NSCs and of intercellular communications among non-irradiated and irradiated NSCs and tumor cells. Here, we investigated the effects of gamma-ray and carbon-ion irradiation on the growth and interphase death of NSCs and glioblastoma cells. NSCs derived from human H9 embryonic stem cells and A172 cells derived from human glioblastoma were irradiated with gamma-rays (60Co, LET = 0.2 keV/um) at the Cobalt 60 Irradiation Facilities and with carbon-ion beam (12C5+, 18.3 MeV/n, LET = 108 keV/um) at the HY1 port of TIARA. After irradiation, cells were incubated for 72 h and, then, stained with cell-membrane permeable and impermeable DNA-binding dyes to discriminate living and dead cells. The numbers of living and dead cells were measured using a flow cytometer. Growth rates were obtained by dividing the number of total (living & dead) cells after 72 h post-irradiation incubation by that of total cells just before irradiation. Survival rates were obtained as the percentage of living cells. Growth rates decreased in dose-dependent manners after carbon-ion and gamma-ray irradiation both in NSCs and A172 cells, meaning induction of interphase death and/or reproductive death. The percentages of living cells were measured to investigate the contribution of interphase death to decrease in growth rates. Survival rates decreased clearly in NSCs after irradiation but not in A172 cells. Our results suggest that growth rates were decreased by different mechanisms in NSCs and A172 cells, and that interphase death and reproductive death are likely main causes in NSCs and A172 cells, respectively. |
|||||
| 書誌情報 |
QST Takasaki Annual Report 2015 p. 110-110, 発行日 2017-03 |
|||||
| 出版者 | ||||||
| 出版者 | Takasaki Advanced Radiation Research Institute, QST | |||||
