WEKO3
アイテム
BPA Concentration-Dependency of HSG Cell Inactivation by Neutron Irradiation
https://repo.qst.go.jp/records/55898
https://repo.qst.go.jp/records/55898a6f6574a-7204-44ef-b865-76f29e4e95a7
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-12-17 | |||||
| タイトル | ||||||
| タイトル | BPA Concentration-Dependency of HSG Cell Inactivation by Neutron Irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
平山, 亮一
× 平山, 亮一× 松本, 孔貴× 増永, 慎一郞× 櫻井, 良憲× 田中, 浩基× 古澤, 佳也× 平山 亮一× 松本 孔貴× 古澤 佳也 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | INTRODUCTION: Excellent dose distribution of neu-tron capture reaction of boron atom induces high relative biological effectiveness (RBE) and the low oxygen en-hancement ratio (OER). These phenomena are com-monly assumed to be an interaction between cells and low energy heavy particles (α and Li) resulting from the boron atom fissions in the cells. However, there has been little study done concerning the action of the parti-cles on living cells. We have investigated contributions of indirect actions of radiation in cell killing by heavy ions with radical scavenger that selectively reduces the indirect action [1-3]. Therefore, it is important that how these mechanisms can be made to clear through a thorough basic research in boron neutron capture therapy is urgently discussed. The main object of this year is to make clear an influ-ence of boron neutron capture reaction at high concen-tration of 10B-para-boronophenylalanine (BPA) on HSG cell inactivation. \n EXPERIMENTS: Human salivary gland tumor (HSG) cells were grown in Eagle’s minimum essential medium supplemented with 10% fetal bovine serum and antibi-otics (100 U/ml penicillin and 100 µg/ml streptomycin) under humidified air with 5% CO2 at 37˚C. The HSG cells were incubated with 25 µg/ml BPA for 3 h before irradiation. The cells were suspended at a density of about 7 × 105 cells/ml containing 25 µg/ml BPA. The cells in Polypropylene tubes (NUNC) were irradiated at the remodeled heavy water facility at the KURRI. Total fluencies of thermal neutron, epithermal neutron and fast neutron were measured by means of gold foil activation analysis. The gamma ray dose including secondary gamma rays was measured with a thermo luminescence dosimeter. Boron concentrations in the cells were taken to be equivalent to those in the medium as reported previously [4]. After irradiation, cells were seeded in triplicate onto 60 mm (Φ) culture dishes at densities to give approximately 100 colonies per dish. After 14 days of incubation, the colonies were fixed with 10 % formalin solution and stained with 1 % methylene blue in water. Colonies consisting of more than 50 surviving cells were scored. The survival curves were fitted by the single-hit model: SF = exp (-α∙D). SF and D are the surviving fraction and the dose (Gy-1), respectively. \n RESULTS: The survival curves for neutrons are shown in Fig. 1. The D10 value of neutron with and without BPA were 0.2 and 2.8, respectively. The D10 value of X-rays was 4.7 Gy. The RBE values for with and with-out BPA were 19.9 and 1.7, respectively. The survival curves of the coefficient of determination (R2) were above 0.8. We will retest and add the dose points and BPA concentrations. These results suggest that he large RBE was induced by low energy heavy particles (α and Li) from boron neutron capture reaction. \n \n REFERENCES: [1] A. Ito et al., Radiat. Res., 165 (2006) 703-712. [2] R. Hirayama et al., Radiat. Res., 171 (2009) 212-218. [3] R. Hirayama et al., Radiat. Phys. Chem., 78 (2009) 1175-1178. [4] Y. Sakurai and T. kobayashi, Nucl. Instrum. Methods Phys. Res. Sect. A., 453 (2000) 569-596. |
|||||
| 書誌情報 |
KURRI Progress Report 2012 p. 151, 発行日 2013-10 |
|||||
