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重粒子線誘発DNA 障害の特徴
https://repo.qst.go.jp/records/55617
https://repo.qst.go.jp/records/5561700e29245-397b-4865-abc3-7baf0c36372e
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-09-30 | |||||
| タイトル | ||||||
| タイトル | 重粒子線誘発DNA 障害の特徴 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
笠井, 清美
× 笠井, 清美× 辻田, 瑛那× 森, 雅彦× 笠井 清美× 辻田 瑛那× 森 雅彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We studied the localization of phosphorylated H2AX (gamma-H2AX) in cultured human fibroblasts (NB1RGB) and EYFP-tagged rad51 in Chinese hamster CHO cells after irradiation with heavy ion beams. Asynchronous cells were irradiated with X-rays, carbon ion beam (LET is about 30 keV/µm and 85 keV/µm), Si ion beam (220 keV/µm) and Fe ion beam (440 keV/µm) at room temperature. The amount of gamma-H2AX monitored by flow cytometry increased just after irradiation of each radiation and reached maximum around 30 min. It was then decreased quickly for cells irradiated with X-rays but presented longer for Si or Fe beams. Average amount of gamma-H2AX per cells at 30 min after irradiation increased with dose for all radiations tested and was similar between Si and Fe beams and was bigger than X-rays. Under the confocal microscopy, foci of gamma-H2AX on cell nucleus was not visible just after X-irradiation, but obvious after Fe ion irradiation. Number of foci per nucleus was increased with increasing dose at 30 min after irradiation similarly for all radiation tested. On the other hand, rad51 foci were not visible just after irradiation and observed about 1 h after irradiation and were visible up to 10 h after irradiation. | |||||
| 書誌情報 |
放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書 巻 HIMAC-125, p. 94-95, 発行日 2007-06 |
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| 出版者 | ||||||
| 出版者 | 放医研 | |||||
