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In vitro analysis of redox status of normal and tumor cells using nitroxide radicals and EPR spectroscopy
https://repo.qst.go.jp/records/54515
https://repo.qst.go.jp/records/545159bc15d7d-2155-4073-b013-95a653ca0094
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-21 | |||||
| タイトル | ||||||
| タイトル | In vitro analysis of redox status of normal and tumor cells using nitroxide radicals and EPR spectroscopy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Georgieva, Ekaterina
× Georgieva, Ekaterina× Ivanova, Donika× Lazarova, Desislava× Bakalova-Zheleva, Rumiana× Aoki, Ichio× Zhelev, Zhivko× Ｇｅｏｒｇｉｅｖａ Ｅｋａｔｅｒｉｎａ× イヴァノヴァ ドニカ× ラザロヴァ デシスラヴァ× バカロバ ルミアナ× 青木 伊知男× Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In present study we describe in vitro imaging analysis of cellular redox status in cancer and noncancer cells, using two redox-sensitive nitroxide derivatives (methoxy-TEMPO and mito-TEMPO) and electron paramagnetic resonance (EPR) spectroscopy. The nitroxide radical (which is characterized by EPR contrast) participates in electron-transfer reactions with variety of intracellular oxidative and reducing equivalents. The rate constants of these reactions determine the EPR signal dynamics in cell suspension. In non-cancer cells, EPR signal intensity of nitroxides decreased significantly during incubation. In cancer cells, EPR signal intensity was almost constant within two hours incubation and did not decreased significantly. The data suggest that non-cancer cells are characterized by a higher reducing activity to the nitroxide radical, while cancer cells are characterized by a higher oxidative activity. The described methodology is appropriate sensing platform for differentiation of cancer from non-cancer cells based on their redox status. | |||||
| 書誌情報 |
Anticancer Research 巻 34, p. 5917-5919, 発行日 2014-10 |
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| 出版者 | ||||||
| 出版者 | International Institute of Anticancer Research (IIAR) | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0250-7005 | |||||
