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Repair pathways for heavy ion-induced complex DNA double strand breaks
https://repo.qst.go.jp/records/54418
https://repo.qst.go.jp/records/544183bbbf3bc-4778-4c0f-a5d3-29256ab3f6e2
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-12-03 | |||||
| タイトル | ||||||
| タイトル | Repair pathways for heavy ion-induced complex DNA double strand breaks | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
矢島, 浩彦
× 矢島, 浩彦× 中島, 菜花子× 平川, 博一× 村上, 健× 藤森, 亮× 岡安, 隆一× 矢島 浩彦× 中島 菜花子× 平川 博一× 村上 健× 藤森 亮× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: The National Institute of Radiological Sciences (NIRS) started heavy ion cancer therapy in 1994 and has treated more than 6000 patients with satisfactory results. It is thought complex DNA double strand breaks (DSBs) induced by high linear energy transfer (LET) heavy ions would cause effective tumor cell killing. However, cellular/molecular responses to complex DSBs are not fully investigated. With the hope of further improvement in heavy ion cancer therapy, we studied the repair process of DSBs induced by heavy ions at the molecular level. Materials and Methods: Human tumor cells were irradiated with X-rays and heavy ions. DNA breaks were analyzed by constant field gel electrophoresis. Cell survival was measured by the conventional colony formation assay. Western blot was used for protein expression analysis including phosphorylated proteins. Immunofluorescence was used to observe cellular protein expression levels with specific primary antibodies and fluorescence-conjugated secondary antibodies. Results and Conclusion: Our data showed that rejoining of DSBs induced by carbon ions was significantly impaired when compared to X-rays. Further, we found that CtIP was intensely phosphorylated after heavy-ion irradiation especially at early time points. This suggests that complex DSBs effectively evokes DNA end resection activity for homologous recombination, in which CtIP has a critical role. This was supported by a markedly elevated RPA2 phosphorylation level, another good indicator for end resection. Our results suggest that the homologous recombination pathway would be a good target for radio-sensitization particularly in heavy-ion therapy. |
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| 書誌情報 |
Proceedings of International Conference on Emerging Frontiers & Challenges in Radiation Biology 発行日 2013-07 |
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