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Repair of DNA double strand breaks induced by heavy ion irradiation
https://repo.qst.go.jp/records/54228
https://repo.qst.go.jp/records/542282f4efd9c-2bda-4fcb-a164-14dd120b36db
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-06-25 | |||||
| タイトル | ||||||
| タイトル | Repair of DNA double strand breaks induced by heavy ion irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Okayasu, Ryuichi
× Okayasu, Ryuichi× Kato, Takamitsu× Fujimori, Akira× Noguchi, Miho× Fujii, Yoshihiro× 岡安 隆一× 加藤 宝光× 藤森 亮× 野口 実穂× 藤井 義大 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | At the National Institute of Radiological Sciences (NIRS), over 4000 cancer patients have been treated by heavy ions with an impressive success rate. There are clinical, physical and biological factors behind this achievement, and in this presentation a few critical biological issues are discussed. Besides the physical advantage of the dose distribution of heavy ions, two important biological factors exist; one of them the inhibition of DNA double-strand break (DSB) repair by heavy ions and the other the reduced variation of cell survival levels throughout the cell cycle. The repair of DNA DSB was examined using constant field gel electrophoresis as well as gamma-H2AX assay, and both techniques clearly showed a significant inhibition of initial rejoining of DSBs induced by 70 keV/µm carbon ions. The degree of repair inhibition seems to be reflected in the radiation cell survival level. Using synchronized CHO cells, we investigated cell survival levels throughout the cell cycle with carbon ions, and the result was compared with that with X-rays. Our data indicated that much less variation in the cell survival level was observed when carbon ions were used to irradiate cells. Moreover, gene expression studies using a unique method developed at NIRS provided new insight into biological damage induced by heavy ions. These data together provide biological reasons for the successful outcome reported at the heavy ion facility at NIRS | |||||
| 書誌情報 |
NIRS-IMP Joint Symposium on Carbon Ion Therapy p. 87-92, 発行日 2009-08 |
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| 出版者 | ||||||
| 出版者 | National Institute of Radiological Science | |||||
