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Transcription factors binding to the cis-elements of GADD45 gene in ML-1 cells after low-dose irradiation.
https://repo.qst.go.jp/records/53234
https://repo.qst.go.jp/records/532343dff1813-7ce0-41fb-979a-09b8f75bb459
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-11-14 | |||||
| タイトル | ||||||
| タイトル | Transcription factors binding to the cis-elements of GADD45 gene in ML-1 cells after low-dose irradiation. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Daino, Kazuhiro
× Daino, Kazuhiro× Ichimura, Sachiko× Nenoi, Mitsuru× 臺野 和広× 沼田 幸子× 根井 充 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Several stress-responsive genes, including p53-target genes, are induced by low-dose of ionizing radiation ranging from 0.02 to 0.5 Gy. We show here that 0.5 Gy of X-rays increase the transcription rate of the GADD45 gene, with maximum induction at 0.5 to 1 h after irradiation, much earlier than the maximum accumulation of stabilized p53 protein. It could be suggested that some transcription factors cooperate with p53 in regulating the GADD45 gene at an early time after low-dose irradiation. This idea is supported by the studies that showed GKLF and Sp1 are required for p53-dependent transcriptional activation of the p21WAF1/Cip1and BAX genes, respectively. To examine the possible involvement of cooperating transcription factors in regulation of the GADD45 gene after low-dose radiation, we attempted a comprehensive EMSA, in which 136 species of double-stranded DNA probes were used to identify X-ray-inducible factor-bindings to the upstream and the third intron regions of the gene after exposure to 0.5 Gy of X-rays in human myeloblastic leukemia ML-1 cells. Several X-ray-inducible DNA-protein complexes were observed. The factors related to forkhead transcription factors, POU domain transcription factors and kruppel-like factors were putatively identified by the competition assay. It is possible that these factors cooperate with p53 to mediate the transcriptional regulation of the GADD45 genes after low-dose irradiation. | |||||
| 書誌情報 |
Molecular Mechanisms for Radiation-Induced Cellular Response and Cancer Development : Proceeding of the International Symposium on Biological Effects of Low Dose Radiation p. 234-239, 発行日 2002 |
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| 出版者 | ||||||
| 出版者 | Institute for Environmental Science | |||||
