WEKO3
アイテム
One-pot synthesis of highly dispersible fluorescent nanodiamonds for bioconjugation
https://repo.qst.go.jp/records/49399
https://repo.qst.go.jp/records/49399583883bb-4142-482b-b2ff-2b1aa27a761d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-07-06 | |||||
| タイトル | ||||||
| タイトル | One-pot synthesis of highly dispersible fluorescent nanodiamonds for bioconjugation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Terada, Daiki
× Terada, Daiki× Sotoma, Shingo× Harada, Yoshie× Igarashi, Ryuji× Shirakawa, Masahiro× Harada, Yoshie× Igarashi, Ryuji× Shirakawa, Masahiro |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Fluorescent nanodiamonds (FNDs) have been attracting much attention as promising therapeutic agents and probes for bioimaging and nanosensing. For their biological applications, several hydrophilizing methods to enhance FND colloidal stability have been developed to suppress their aggregation and the nonspecific adsorption to biomolecules in complex biomedical environments. However, these methods involve several complicated synthetic and purification steps, which prohibit the use of FNDs for bioapplications by biologists. In this study, we describe a simple one-pot FND hydrophilization method that comprises coating of the surface of the nanoparticles with COOH-terminated hyperbranched polyglycerol (HPG-COOH). HPG-COOH-coated FNDs (FND-HPG-COOHs) were found to exhibit excellent dispersibility under physiological conditions despite the thinness of 5-nm HPG-COOH layer. Biotinylated FND-HPG-COOHs specifically captured avidin molecules in the absence of nonspecific protein adsorption. Moreover, we demonstrated that FND-HPG-COOHs conjugated with antibodies can be used to selectively target integrins in fixed HeLa cells. In addition, intracellular temperature changes were measured via optically detected magnetic resonance using FND-HPG-COOHs conjugated with mitochondrial localization signal peptides. Our one-pot synthetic method will encourage the broad use of FNDs among molecular and cellular biologists, and pave the way for extensive biological and biomedical applications of FNDs. | |||||
| 書誌情報 |
Bioconjugate Chemistry 巻 29, 号 8, p. 2786-2792, 発行日 2018-07 |
|||||
| 出版者 | ||||||
| 出版者 | ACS publications | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1043-1802 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1021/acs.bioconjchem.8b00412 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.8b00412 | |||||
