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Quantitative analysis of mammalian chromosome by scanning transmission soft X-ray microscopy
https://repo.qst.go.jp/records/49108
https://repo.qst.go.jp/records/4910840c474d5-882d-44e2-89f7-b95cb27f4761
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-07-20 | |||||
| タイトル | ||||||
| タイトル | Quantitative analysis of mammalian chromosome by scanning transmission soft X-ray microscopy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shinohara, K.
× Shinohara, K.× Ohigashi, T.× Tone, S.× Kado, M.× Ito, A.× 加道 雅孝 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Soft X-ray spectromicroscopy was applied to study the quantitative distribution of DNA and protein in a mammalian chromosome at the spatial resolution of 100 nm. The quantities of DNA and protein were evaluated using 1s-π* transition in the NEXAFS spectra at the nitrogen K absorption edge. DNA was not uniformly distributed in the chromosome and DNA/protein ratio was less than 0.497. The present analysis revealed the clues to identify other molecules that contribute to the absorption spectrum of the sample. The results suggested that accumulation of the absorption spectra of relevant molecules would support the refinement of the analysis. | |||||
| 書誌情報 |
Ultramicroscopy 巻 194, p. 1-6, 発行日 2018-07 |
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| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.ultramic.2018.07.001 | |||||
