WEKO3
アイテム
Cloning of canine Ku80 and its localization and accumulation at DNA damage sites
https://repo.qst.go.jp/records/49019
https://repo.qst.go.jp/records/49019fba15c56-d116-4e48-8658-6e5aa2d622c8
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-05-16 | |||||
| タイトル | ||||||
| タイトル | Cloning of canine Ku80 and its localization and accumulation at DNA damage sites | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
小池, 学
× 小池, 学× 湯徳, 靖友× 小池, 亜紀× 小池 学× 湯徳 靖友× 小池 亜紀 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Molecularly targeted therapies have high specificity and significant cancer- killing effect. However, their antitumor effect might be greatly diminished by variation in even a single amino acid in the target site, as it occurs, for example, as a consequence of SNPs. Increasing evidence suggests that the DNA repair protein Ku80 is an attractive target molecule for the develop- ment of next-generation radiosensitizers for human cancers. However, the localization, post-translational modifications (PTMs), and complex forma- tion of Ku80 have not been elucidated in canines. In this study, for the first time, we cloned, sequenced, and characterized canine Ku80 cDNA. Our data show that canine Ku80 localizes in the nuclei of interphase cells and is quickly recruited at laser-induced double-strand break sites. Comparative analysis shows that canine Ku80 had only 82.3% amino acid identity with the homologous human protein, while the nuclear localization signal (NLS) in human and canine Ku80 is evolutionarily conserved. Notably, some pre- dicted PTM sites, including one acetylation site and one sumoylation site within the NLS, are conserved in the two species. These findings suggest that the spatial and temporal regulation of Ku80 might be conserved in humans and canines. However, our data indicate that the expression of Ku80 is considerably lower in the canine cell lines examined than in human cell lines. These important findings might be useful to better understand the mechanism of the Ku80-dependent DNA repair and for the develop- ment of potential next-generation radiosensitizers targeting common targets in human and canine cancers. | |||||
| 書誌情報 |
FEBS Open Bio (Online Only URL:http://www.sciencedirect.com/science/journal/22115463) 巻 7, 号 12, p. 1854-1863, 発行日 2017-11 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2211-5463 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/2211-5463.12311 | |||||
