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Region-specific irradiation system with heavy-ion microbeam for active individuals of Caenorhabditis elegans
https://repo.qst.go.jp/records/48951
https://repo.qst.go.jp/records/48951aee93d5d-7457-479c-a674-369974a0c583
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-05-11 | |||||
| タイトル | ||||||
| タイトル | Region-specific irradiation system with heavy-ion microbeam for active individuals of Caenorhabditis elegans | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suzuki, Michiyo
× Suzuki, Michiyo× Hattori, (Tokyo Institute of Technology) Yuya× Sakashita, Tetsuya× Yokota, Yuichiro× Kobayashi, Yasuhiko× Funayama, Tomoo× 鈴木 芳代× 坂下 哲哉× 横田 裕一郎× 小林 泰彦× 舟山 知夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radiation may affect essential functions and behaviors such as locomotion, feeding, learning, and memory. Although whole-body irradiation has been shown to reduce motility in the nematode Caenorhabditis elegans, the detailed mechanism responsible for this effect remains unknown. Targeted irradiation of the nerve ring responsible for sensory integration and information processing would allow us to determine if the reduction of motility following whole-body irradiation reflects effects on the central nervous system or on the muscle cells themselves. We therefore addressed this issue using a collimating microbeam system. However, radiation targeting requires the animal to be immobilized, and previous studies have anesthetized animals to prevent their movement, thus making it impossible to assess their locomotion immediately after irradiation. We developed a method in which the animal was enclosed in a straight, microfluidic channel in a polydimethylsiloxane chip to inhibit free motion during irradiation, thus allowing locomotion to be observed immediately after irradiation. The head region, including the central nervous system, mid region around the intestine and uterus, and tail region were targeted independently. Each region was irradiated with 12,000 carbon ions (12C; 18.3 MeV/u; linear energy transfer = 106.4 keV/μm) corresponding to 500 Gy at a φ20 μm region. Motility was significantly decreased by whole-body irradiation, but not by irradiation of any of the individual regions, including the central nervous system. This suggests that radiation inhibits locomotion by a whole-body mechanism, potentially involving motoneurons and/or body-wall muscle cells, rather than affecting motor control via the central nervous system and the stimulation response. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 58, 号 6, p. 881-886, 発行日 2017-09 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/jrr/rrx043 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1093/jrr/rrx043 | |||||
| 関連名称 | https://doi.org/10.1093/jrr/rrx043 | |||||
