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Structural Basis of Homology-Directed DNA Repair Mediated by RAD52
https://repo.qst.go.jp/records/48838
https://repo.qst.go.jp/records/488382a279e4f-d842-4849-83d9-a5a9cf9d73f4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-05-07 | |||||
| タイトル | ||||||
| タイトル | Structural Basis of Homology-Directed DNA Repair Mediated by RAD52 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Saotome, Mika
× Saotome, Mika× Saito, Kengo× 安田, 武嗣× Ohtomo, Hideaki× Sugiyama, Shusei× Nishimura, Yoshifumi× Kurumizaka, Hitoshi× Kagawa, Wataru× 安田 武嗣 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | RAD52 mediates homologous recombination by annealing complementary DNA strands. However, the detailed mechanism of DNA annealing promoted by RAD52 has remained elusive. Here we report two crystal structures of human RAD52-single-stranded DNA (ssDNA) complexes that probably represent key reaction intermediates of RAD52-mediated DNA annealing. The first structure revealed a ‘wrapped’ conformation of ssDNA around the homo-oligomeric RAD52 ring, in which the edges of the bases involved in base pairing are exposed to the solvent. The ssDNA conformation is close to B-form, and appears capable of engaging in Watson-Crick base pairing with the complementary DNA strand. The second structure revealed a ‘trapped’ conformation of ssDNA between two RAD52 rings. This conformation is stabilized by a different RAD52 DNA binding site, which promotes the accumulation of multiple RAD52 rings on ssDNA and the aggregation of ssDNA. These structures provide a structural framework for understanding the mechanism of RAD52-mediated DNA annealing. | |||||
| 書誌情報 |
iScience 巻 3, p. 50-62, 発行日 2018-05 |
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| 出版者 | ||||||
| 出版者 | Cell Press | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.isci.2018.04.005 | |||||
