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Positron emission tomography of cerebral angiogenesis and TSPO expression in a mouse model of chronic hypoxia.
https://repo.qst.go.jp/records/48814
https://repo.qst.go.jp/records/48814b49fd7d5-0696-4e8a-8b57-4052a612393c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-04-26 | |||||
| タイトル | ||||||
| タイトル | Positron emission tomography of cerebral angiogenesis and TSPO expression in a mouse model of chronic hypoxia. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kanno, Iwao
× Kanno, Iwao× Seki, Chie× Takuwa, Hiroyuki× Jin, Zhao-Hui× Boturyn, Didier× Dumy, Pascal× Furukawa, Takako× Saga, Tsuneo× Ito, Hiroshi× Masamoto, Kazuto× 菅野 巖× 関 千江× 田桑 弘之× 金 朝暉× 古川 高子× 佐賀 恒夫× 伊藤 浩× 正本 和人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The present study aimed to examine whether positron emission tomography (PET) could evaluate cerebral angiogenesis. Mice were housed in a hypoxic chamber with 8-9% oxygen for 4, 7, and 14 days, and the angiogenic responses were evaluated with a radiotracer, (64)Cu-cyclam-RAFT-c(-RGDfK-)4, which targeted αVβ3 integrin and was imaged with PET. The PET imaging results showed little uptake during all of the hypoxic periods. Immunofluorescence staining of the β3 integrin, CD61, revealed weak expression, while the microvessel density assessed by CD31 staining increased with the hypoxic duration. These observations suggest that the increased vascular density originated from other types of vascular remodeling, unlike angiogenic sprouting. We then searched for any signs of vascular remodeling that could be detected using PET. PET imaging of (11)C-PK11195, a marker of the 18-kDa translocator protein (TSPO), revealed a transient increase at day 4 of hypoxia. Because the immunofluorescence of glial markers showed unchanged staining over the early phase of hypoxia, the observed upregulation of TSPO expression probably originated from non-glial cells (e.g. vascular cells). In conclusion, a transient increase in TSPO probe uptake was detected with PET at only the early phase of hypoxia, which indicates an early sign of vascular remodeling induced by hypoxia. | |||||
| 書誌情報 |
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 巻 38, 号 4, p. 687-696, 発行日 2017-01 |
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| 出版者 | ||||||
| 出版者 | SAGE Publications | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0271-678X | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 28128020 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1177/0271678X16689800 | |||||
