WEKO3
アイテム
Investigation of the relative biological effectiveness and uniform isobiological killing effects of irradiation with a clinical carbon SOBP beam on DNA repair deficient CHO cells
https://repo.qst.go.jp/records/48812
https://repo.qst.go.jp/records/48812b7a07313-c573-4c70-8175-981b731991b6
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-04-25 | |||||
| タイトル | ||||||
| タイトル | Investigation of the relative biological effectiveness and uniform isobiological killing effects of irradiation with a clinical carbon SOBP beam on DNA repair deficient CHO cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sunada, Shigeaki
× Sunada, Shigeaki× M., Cartwright Ian× Hirakawa, Hirokazu× Fujimori, Akira× Uesaka, Mitsuru× Kato, Takamitsu× 砂田 成章× 平川 博一× 藤森 亮× 上坂 充× 加藤 宝光 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Spread-out Bragg peak (SOBP) C ions have been used clinically in charged particle radiation therapy for years. An SOBP beam consists of various monoenergetic Bragg peaks; however, the biological effect of irradiation with an SOBP beam track has not been well-studied. In order to determine the clinically prospective molecular targets, radiosensitivity to the beam track in DNA repair de cient cell lines was investi- gated. A total of four distinct Chinese hamster ovary (CHO) cell lines, including CHO10B2 (wild-type), V3 (protein kinase DNA‐activated catalytic polypeptide de cient), 51D1 (RAD51 paralog D deficient) and PADR9 [poly(ADP-ribose) poly- merase (PARP) de cient], were irradiated with gamma-rays, C ions (290 MeV/n) and Fe ions (500 MeV/n), in order to compare cellular lethality. An OptiCellTM culture system was used to evaluate the lethality at distinct depths of SOBP C ions. Relative biological effectiveness (RBE) values of C ions (linear energy transfer (LET), 13 and 70 keV/μm) and Fe ions (LET, 200 keV/μm) were calculated from cell survival using colony formation assay with standard cell dishes. All cell lines exhibited similar sensitivity to 70 keV/μm C ions and 200 keV/ μm Fe ions. Furthermore, V3 cells did not exhibit increased sensitivity to high LET C ions and Fe ions, compared with low LET gamma‐rays and C ions, and 51D1 cells irradiated with 13 keV/μm C ions exhibited relatively high RBE values among the tested cell lines. Conversely, PADR9 cells exhibited low RBE values for 13 keV/μm C ions and high RBE values for 70 keV/μm C ions. Obtained using the OptiCell system, the survival fractions in the SOBP region were uniform for wild‐type and PADR9 cells. Conversely, V3 and 51D1 cells exhibited decreased cell death in the distal region of the SOBP. These results indicated that PARP is a more effective target for clinical beam therapy, compared with the non-homologous end joining repair and homologous recombination repair pathways. PARP de ciency may be an optimal target for C ion therapy and the results of the present study may contribute to the development of a more effective heavy ion radiation therapy. | |||||
| 書誌情報 |
ONCOLOGY LETTERS 巻 13, 号 6, p. 4911-4916, 発行日 2017-04 |
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| 出版者 | ||||||
| 出版者 | SPANDIDOS PUBLICATIONS | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 28599495 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3892/ol.2017.6072 | |||||
