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3D reconstruction of brain section images for creating axonal projection maps in marmosets
https://repo.qst.go.jp/records/48774
https://repo.qst.go.jp/records/48774f128016e-fb8d-4b52-a9d7-5b9894023ca8
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-04-23 | |||||
| タイトル | ||||||
| タイトル | 3D reconstruction of brain section images for creating axonal projection maps in marmosets | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Abe, Hiroshi
× Abe, Hiroshi× Tani, Toshiki× Mashiko, Hiromi× Kitamura, Naohito× Miyakawa, Naohisa× Mimura, Koki× Sakai, Kazuhisa× Suzuki, Wataru× Kurotani, Tohru× Mizukami, Hiroaki× Watakabe, Akiya× Yamamori, Tetsuo× Ichinohe, Noritaka× 宮川 尚久× 三村 喬生× 鈴木 航× 一戸 紀孝 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | BACKGROUND: The brain of the common marmoset (Callithrix jacchus) is becoming a popular non-human primate model in neuroscience research. Because its brain fiber connectivity is still poorly understood, it is necessary to collect and present connection and trajectory data using tracers to establish a marmoset brain connectivity database. NEW METHOD: To visualize projections and trajectories of axons, brain section images were reconstructed in 3D by registering them to the corresponding block-face brain images taken during brain sectioning. During preprocessing, autofluorescence of the tissue was reduced by applying independent component analysis to a set of fluorescent images taken using different filters. RESULTS: The method was applied to a marmoset dataset after a tracer had been injected into an auditory belt area to fluorescently label axonal projections. Cortical and subcortical connections were clearly reconstructed in 3D. The registration error was estimated to be smaller than 200 μm. Evaluation tests on ICA-based autofluorescence reduction showed a significant improvement in signal and background separation. COMPARISON WITH EXISTING METHODS: Regarding the 3D reconstruction error, the present study shows an accuracy comparable to previous studies using MRI and block-face images. Compared to serial section two-photon tomography, an advantage of the proposed method is that it can be combined with standard histological techniques. The images of differently processed brain sections can be integrated into the original ex vivo brain shape. CONCLUSIONS: The proposed method allows creating 3D axonal projection maps overlaid with brain area annotations based on the histological staining results of the same animal. |
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| 書誌情報 |
Journal of neuroscience methods 巻 286, 号 286, p. 102-113, 発行日 2017-05 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0165-0270 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 28577985 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.jneumeth.2017.04.016 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.sciencedirect.com/science/article/pii/S0165027017301152 | |||||
| 関連名称 | http://www.sciencedirect.com/science/article/pii/S0165027017301152 | |||||
