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Crystallographic characterization of the high-potential iron-sulfur protein in the oxidized state at 0.8 A resolution
https://repo.qst.go.jp/records/48753
https://repo.qst.go.jp/records/48753d09e450a-9fc3-47dc-92bc-005f362dd3e3
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-04-19 | |||||
| タイトル | ||||||
| タイトル | Crystallographic characterization of the high-potential iron-sulfur protein in the oxidized state at 0.8 A resolution | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hiraku, Ohno
× Hiraku, Ohno× Takeda, Kazuki× Niwa, Satomi× Tsujinaka, Tomotaka× Hanazono, Yuya× 平野, 優× Miki, Kunio× 平野 優 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | High-potential iron-sulfur protein (HiPIP) is a soluble electron carrier protein of photosynthetic bacteria with an Fe4S4 cluster. Although structural changes accompanying the electron transfer are important for understanding of the functional mechanism, the changes have not been clarified in sufficient detail. In the present study, we performed a crystallographic analysis of oxidized HiPIP and a structural comparison with the reduced form at a high resolution of 0.8 Å. The changes in Fe-S bond lengths were similar to that predicted by theoretical calculation, although some discrepancies were also found. Almost distances between the sulfur atoms of the iron-sulfur cluster and the protein environment are elongated upon the oxidation. Positional changes of hydrogen atoms in the protein environment, such as on the amide-hydrogen of Cys75 in the proximity of the iron-sulfur cluster, were also observed in the accurate analyses. None of the water molecules exhibited significant changes in position or anisotropy of atomic displacement parameter between the two states, while the orientations of some water molecules were different. | |||||
| 書誌情報 |
PLoS ONE 巻 12, 号 5, p. e0178183, 発行日 2017-05 |
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| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 28542634 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pone.0178183 | |||||
