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2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching
https://repo.qst.go.jp/records/48661
https://repo.qst.go.jp/records/486610b871f35-710b-4061-b4ef-a8d7ff8eadf0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-04-06 | |||||
| タイトル | ||||||
| タイトル | 2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Atsushi, Matsumoto
× Atsushi, Matsumoto× Naoyuki, Miyazaki× Junichi, Takagi× Kenji, Iwasaki× 松本 淳 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In this study, we develop an approach termed “2D hybrid analysis” for building atomic models by image matching from electron microscopy (EM) images of biological molecules. The key advantage is that it is applicable to flexible molecules, which are difficult to analyze by 3DEM approach. In the proposed approach, first, a lot of atomic models with different conformations are built by computer simulation. Then, simulated EM images are built from each atomic model. Finally, they are compared with the experimental EM image. Two kinds of models are used as simulated EM images: the negative stain model and the simple projection model. Although the former is more realistic, the latter is adopted to perform faster computations. The use of the negative stain model enables decomposition of the averaged EM images into multiple projection images, each of which originated from a different conformation or orientation. We apply this approach to the EM images of integrin to obtain the distribution of the conformations, from which the pathway of the conformational change of the protein is deduced. | |||||
| 書誌情報 |
Scientific reports 巻 7, p. 377-1-377-12, 発行日 2017-03 |
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| 出版者 | ||||||
| 出版者 | Nature Publishing Group | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2045-2322 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/s41598-017-00337-y | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | www.nature.com/articles/s41598-017-00337-y | |||||
| 関連名称 | www.nature.com/articles/s41598-017-00337-y | |||||
