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Expression and characterization of the Renilla luciferase with the cumulative mutation
https://repo.qst.go.jp/records/48547
https://repo.qst.go.jp/records/4854788f7b716-ca23-4e95-800f-3af7eea1d503
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-02-28 | |||||
| タイトル | ||||||
| タイトル | Expression and characterization of the Renilla luciferase with the cumulative mutation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matsujiro, Ishibashi（鹿児島大学農学部）
× Matsujiro, Ishibashi（鹿児島大学農学部）× Ryo, Kawanabe（鹿児島大学農学部）× Norie, Amaba（鹿児島大学農学部）× Arai, Shigeki× Fina, Amreta Laksmi（鹿児島大学農学部）× Kenta, Komori（鹿児島大学農学部）× Tokunaga, Masao× 新井 栄揮 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Luciferase from Renilla reniformis (RLuc) is a good research tool as a reporter protein and bioimaging probes, yielding blue light using the substrate coelenterazine. However, the applications are limited since RLuc is unstable under various conditions. Therefore, an attempt was made to increase RLuc thermostability. In this study, 5 mutations reported previously [M. Shigehisa et al., Protein Eng. Des. Sel. 30 (2017) 7–13] and one mutation obtained using site-directed mutagenesis were combined. As a result of this combination, the thermostability effect increased, with the mutant showing approximately 10 °C higher stability. Furthermore, the mutant simultaneously improved a tolerance for protease digestion, e.g. trypsin and proteinase K, and for organic solvent. Residual activity of the mutant after treatment with 10% 2-propanol, 10% DMF and 20% DMSO at 35 °C for 1 h was 29.4, 24.8 and 91.3%, respectively, whereas that of the wild type was 0.4, 0.1 and 24.3%, respectively. | |||||
| 書誌情報 |
Protein Expression and Purification 巻 145, p. 39-44, 発行日 2017-12 |
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| 出版者 | ||||||
| 出版者 | Elsevier Inc. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1046-5928 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 29288731 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.pep.2017.12.010 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S1046592817306083 | |||||
| 関連名称 | https://www.sciencedirect.com/science/article/pii/S1046592817306083 | |||||
