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Neutron crystallography of photoactive yellow protein reveals unusual protonation state of Arg52 in the crystal
https://repo.qst.go.jp/records/48476
https://repo.qst.go.jp/records/484763f88d35a-27d8-4e35-83cb-f1e09681865e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-02-07 | |||||
| タイトル | ||||||
| タイトル | Neutron crystallography of photoactive yellow protein reveals unusual protonation state of Arg52 in the crystal | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yonezawa, Kento
× Yonezawa, Kento× Shimizu, Nobutaka× 栗原, 和男× Yamazaki, Yoichi× Kamikubo, Hironari× Kataoka, Mikio× 栗原 和男 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Because of its high pKa, arginine (Arg) is believed to be protonated even in the hydrophobic environment of the protein interior. However, our neutron crystallographic structure of photoactive yellow protein, a light sensor, demonstrated that Arg52 adopts an electrically neutral form. We also showed that the hydrogen bond between the chromophore and Glu46 is a so-called low barrier hydrogen bond (LBHB). Because both the neutral Arg and LBHB are unusual in proteins, these observations remain controversial. To validate our findings, we carried out neutron crystallographic analysis of the E46Q mutant of PYP. The resultant structure revealed that the proportion of the cationic form is higher in E46Q than in WT, although the cationic and neutral forms of Arg52 coexist in E46Q. These observations were confirmed by the occupancy of the deuterium atom bound to the N η1 atom combined with an alternative conformation of the N(η2)D2 group comprising sp2 hybridisation. Based on these results, we propose that the formation of the LBHB decreases the proton affinity of Arg52, stabilizing the neutral form in the crystal. | |||||
| 書誌情報 |
Scientific Reports (Online Only URL:http://www.nature.com/srep/index.html) 巻 7, 号 1, p. 9361-1-9361-10, 発行日 2017-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2045-2322 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 28839266 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/s41598-017-09718-9 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.nature.com/articles/s41598-017-09718-9 | |||||
| 関連名称 | https://www.nature.com/articles/s41598-017-09718-9 | |||||
