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A New Method for the Simultaneous Detection of Mammalian Cells and Ion Tracks on a Surface of CR-39
https://repo.qst.go.jp/records/48450
https://repo.qst.go.jp/records/484501d30124d-5e25-4799-8c91-d06d67bf180c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-02-04 | |||||
| タイトル | ||||||
| タイトル | A New Method for the Simultaneous Detection of Mammalian Cells and Ion Tracks on a Surface of CR-39 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Konishi, Teruaki
× Konishi, Teruaki× Amemiya, Kuniaki× Natsume, Toshiyuki× Takeyasu, Akihiro× Yasuda, Nakahiro× Furusawa, Yoshiya× Hieda, Kotaro× 小西 輝昭× 竹安 明弘× 安田 仲宏× 古澤 佳也× 檜枝 光太郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The geometric locations of ion traversals in mammalian cells constitute important information in the study of heavy ion-induced biological effects. We employed a contact microscopy technique, which was developed for boron imaging in boron neutron capture therapy to the irradiation mammalian cells by low-energy heavy ions. This method enables the simultaneous visualization of mammalian cells as a relief on a plastic track detector, CR-39, and the etch pits which indicate the positions of ion traversals. This technique provides visual geometric information about the cells and ion traversal, without any specially designed devices or microscopes. Only common laboratory equipment, such as a conventional optical microscope, a UV lamp, and commercially available CR-39 is required. To validate this method, CHO-K1 and HeLa cells were cultured on the CR-39 surface and then irradiated with low-energy Ar and Ne ions, respectively. The positions of induced DNA double strand breaks were detected as g-H2AX fluorescent spots, which coincided with the positions of the etch pits in the cell relief image. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 48, 号 3, p. 255-261, 発行日 2007-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 17435376 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1269/jrr.06078 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/jrr/advpub/0/advpub_0704110004/_article | |||||
| 関連名称 | http://www.jstage.jst.go.jp/article/jrr/advpub/0/advpub_0704110004/_article | |||||
