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Visualization of Heavy Ion Tracks by Labeling 3'-OH Termini of Induced DNA Strand Breaks
https://repo.qst.go.jp/records/48446
https://repo.qst.go.jp/records/48446b252824d-1703-4791-bd2f-fc91b84d7da3
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-02-04 | |||||
| タイトル | ||||||
| タイトル | Visualization of Heavy Ion Tracks by Labeling 3'-OH Termini of Induced DNA Strand Breaks | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Konishi, Teruaki
× Konishi, Teruaki× Takeyasu, Akihiro× Natsume, Toshiyuki× Furusawa, Yoshiya× Hieda, Kotaro× 小西 輝昭× 竹安 明弘× 古澤 佳也× 檜枝 光太郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | African green monkey kidney cells, CV-1, were irradiated with Carbon ions (LET: 735 keV/um Argon ions (LET: 3,000 keV/um) to visualize ion tracks through the cell nucleus by labeling the 3'-OH termini result of DNA strand breaks. The 3'-OH termini of DNA were labeled with BrdU-triphosphate catalyzed by TdT. This method of TUNEL (TdT-mediated dUTP Nick End labeling) is based on the specific binding of TdT to 3'-OH termini of DNA. Subsequent immuno-fluorescent staining with the primary monoclonal antibody against BrdU, followed by a secondary antibody of Alexa Fluor 488, was performed to visualize the BrdU labeled DNA termini. Images of the cell nuclei were acquired by confocal laser microscopy. When cell monolayers were irradiated perpendicularly with argon ions, induced DSBs in cell nuclei were identifiable as fluorescent spots. In another irradiation setup, when cells were irradiated at a small angle with incident argon ions, DNA strand breaks were detected as fluorescent stripes across the cell nucleus. These results demonstrate the induction of 3'-OH termini at sites of DNA strand breaks along Argon ion tracks. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 52, 号 4, p. 433-440, 発行日 2011-07 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 21785232 | |||||
