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SPICE-NIRS Microbeam: A Focused Vertical System for Proton Irradiation of a Single Cell for Radiobiological Research
https://repo.qst.go.jp/records/48445
https://repo.qst.go.jp/records/48445387c606b-6a14-46bc-9f61-eca352ce4b17
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-02-04 | |||||
| タイトル | ||||||
| タイトル | SPICE-NIRS Microbeam: A Focused Vertical System for Proton Irradiation of a Single Cell for Radiobiological Research | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Konishi, Teruaki
× Konishi, Teruaki× Oikawa, Masakazu× Suya, Noriyoshi× Ishikawa, Takahiro× Maeda, Takeshi× Kobayashi, Alisa× Shiomi, Naoko× Kodama, Kumiko× Hamano, Tsuyoshi× Takeda, Shino× Isono, Mayu× Hieda, Kotaro× Uchihori, Yukio× Shirakawa, Yoshiyuki× 小西 輝昭× 及川 将一× 酢屋 徳啓× 石川 剛弘× 前田 武× 小林 亜利紗× 塩見 尚子× 児玉 久美子× 濱野 毅× 武田 志乃× 檜枝 光太郎× 内堀 幸夫× 白川 芳幸 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The Single Particle Irradiation system to Cell (SPICE) facility at the National Institute of Radiological Sciences (NIRS) is a focused vertical microbeam system designed to irradiate the nuclei of adhesive mammalian cells with a defined number of 3.4 MeV protons. The approximately 2-micrometer diameter proton beam is focused with a magnetic quadrupole triplet lens and traverses the cells contained in dishes from bottom to top. All procedures for irradiation, such as cell image capturing, cell recognition and position calculation, are automated. The most distinctive characteristic of the system is its stability and high throughput; i.e. 3,000 cells in a 5 mm x 5 mm area in a single dish can be routinely irradiated by the 2-micrometer beam within 15 min (the maximum irradiation speed is 400 cells per minute). The number of protons can be set as low as one, at a precision measured by CR-39 detectors to be 99.0%. A variety of targeting modes such as fractional population targeting mode, multi-position targeting mode for nucleus irradiation and cytoplasm targeting mode are available. As an example of multi-position targeting irradiation of mammalian cells, five fluorescent spots in a cell nucleus were demonstrated using the gamma-H2AX immune-staining technique. The SPICE performance modes described in this paper are in routine use. SPICE is a joint-use research facility of NIRS and its beam times are distributed for collaborative research. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 54, p. 736-747, 発行日 2013-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 23287773 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 101093/jrr/rrs132 | |||||
