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Rapid isolation of murine primary hepatocytes for chromosomal analysis
https://repo.qst.go.jp/records/48339
https://repo.qst.go.jp/records/483398439ef31-684e-4811-afe2-492cdc2ee9fc
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-09-25 | |||||
| タイトル | ||||||
| タイトル | Rapid isolation of murine primary hepatocytes for chromosomal analysis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ariyoshi, Kentaro
× Ariyoshi, Kentaro× Fujishima, Youhei× Miura, Tomisato× Shang, Yi× Kakinuma, Shizuko× Shimada, Yoshiya× Kasai, Kosuke× Nakata, Akifumi× Tachibana, Akira× A., Yoshida Mitsuaki× 尚 奕× 柿沼 志津子× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Primary hepatocyte culture is a crucial tool for investigations of liver function and for evaluating the toxic effects of drugs. In addition, chromosomal analysis of hepatocytes could also prove useful for understanding the mechanisms of hepatocarcinogenesis. However, cultivation of primary hepatocytes for chromosome analysis has been hampered by the specific equipment and skill required to perform the in situ perfusion step necessary for isolation of primary hepatocytes. In the present study, we aimed to establish a simple and efficient method of isolating hepatocytes suitable for chromosome analysis. We performed hepatocyte isolation without using collagenase perfusion, instead digesting liver tissues using collagenase in tubes. In addition, we examined hepatocyte and bone marrow cell (BMC) co-culture and cultivation of hepatocytes with medium containing BMC culture media supernatants. We found that hepatocyte viability and attachment rate were significantly improved, both by co-culture with BMCs and medium containing BMC culture media supernatants, with the later also significantly increasing the mitotic index. Using this simple method of isolation and cultivation we could successfully perform chromosomal analysis of mouse primary hepatocytes. This method has the potential to help understand the mechanisms underlying chromosomal instability mediated hepatocarcinogenesis. | |||||
| 書誌情報 |
In vitro cellular & Developmental Biology- Animal 巻 53, 号 5, p. 474-478, 発行日 2017-02 |
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| 出版者 | ||||||
| 出版者 | Springer | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1543-706X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s11626-017-0132-7 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://link.springer.com/article/10.1007%2Fs11626-017-0132-7 | |||||
| 関連名称 | http://link.springer.com/article/10.1007%2Fs11626-017-0132-7 | |||||
