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Restoration of ATM Expression in DNA-PKcs–Deficient Cells Inhibits Signal End Joining.
https://repo.qst.go.jp/records/48150
https://repo.qst.go.jp/records/481500c0594c9-ea1c-4e52-b74b-4f966872b59f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-07-24 | |||||
| タイトル | ||||||
| タイトル | Restoration of ATM Expression in DNA-PKcs–Deficient Cells Inhibits Signal End Joining. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
A., Neal Jessica
× A., Neal Jessica× Xu, Yao× Abe, Masumi× Hendrickson, Eric× Meek, Katheryn× 安倍 真澄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Unlike most DNA-dependent protein kinase, catalytic subunit (DNA-PKcs)–deficient mouse cell strains, we show in the present study that targeted deletion of DNA-PKcs in two different human cell lines abrogates VDJ signal end joining in episomal assays. Although the mechanism is not well defined, DNA-PKcs deficency results in spontaneous reduction of ATM expression in many cultured cell lines (including those examined in this study) and in DNA-PKcs–deficient mice. We considered that varying loss of ATM expression might explain differences in signal end joining in different cell strains and animal models, and we investigated the impact of ATM and/or DNA-PKcs loss on VDJ recombination in cultured human and rodent cell strains. To our surprise, in DNA-PKcs–deficient mouse cell strains that are proficient in signal end joining, restoration of ATM expression markedly inhibits signal end joining. In contrast, in DNA-PKcs–deficient cells that are deficient in signal end joining, complete loss of ATM enhances signal (but not coding) joint formation. We propose that ATM facilitates restriction of signal ends to the classical nonhomologous end-joining pathway. | |||||
| 書誌情報 |
Journal of Immunology 巻 196, 号 7, p. 3032-3042, 発行日 2016-02 |
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| 出版者 | ||||||
| 出版者 | Baltimore : Williams & Wilkins | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-1767 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 26921311 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.4049/jimmunol.1501654 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.4049/jimmunol.1501654 | |||||
| 関連名称 | https://doi.org/10.4049/jimmunol.1501654 | |||||
