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Characterization of functional regions for nuclear localization of NPAT
https://repo.qst.go.jp/records/47668
https://repo.qst.go.jp/records/4766821d6374f-989d-4e85-9ebc-373c8f777397
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-03-16 | |||||
| タイトル | ||||||
| タイトル | Characterization of functional regions for nuclear localization of NPAT | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sagara, Masashi
× Sagara, Masashi× Takeda, Eri× Nishiyama, Akiyo× Utsumi, Shunsaku× Toyama, Yoshirou× Yuasa, Shigeki× Ninomiya, Yasuharu× Imai, Takashi× 相良 雅史× 二宮 康晴× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | NPAT plays a role in S phase entry as a substrate of cyclin E-CDK2 and activation of histone gene transcription. Although analysis of its sequence indicates that NPAT contains typical nuclear localization signals (NLS) comprising segments of positively charged amino acids, there are currently no experimental data to show that these predictive NLS are functional. To investigate whether these sequences are effective for nuclear transport of NPAT, an NPAT-green fluorescent protein fusion (NP-GFP) was constructed. After transfection of the fusion gene containing the full coding region of NPAT into cultured cells, the NP-GFP product was found exclusively in the nucleus. As expected, some deletion mutants which retain the basic amino acid clusters at the carboxyl terminus also localize the fusion protein in the nucleus. However other fusions, which lacked one of the three basic amino acid-clusters were distributed throughout the nucleus and cytoplasm. Therefore all three clusters of basic residues are necessary for localization of NPAT to the nucleus. However, another sequence outside the carboxyl terminal region functions similar to NLS. Construction of GFP fusions with a series of truncated forms of NPAT indicated that a short peptide sequence consisting of mainly hydrophobic amino acids near the central domain of NPAT also contribute to localizing the protein in the nucleus. | |||||
| 書誌情報 |
Journal of Biochemistry 巻 132, p. 875-879, 発行日 2017-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021-924X | |||||
