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  1. 原著論文

Establishment and evaluation of a new highly metastatic tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and green fluorescent protein.

https://repo.qst.go.jp/records/47426
https://repo.qst.go.jp/records/47426
31ce4b88-5cbf-426e-9460-c5a1a46c138d
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-05-19
タイトル
タイトル Establishment and evaluation of a new highly metastatic tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and green fluorescent protein.
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Sudo, Hitomi

× Sudo, Hitomi

WEKO 474975

Sudo, Hitomi

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B, Tsuji Atsushi

× B, Tsuji Atsushi

WEKO 474976

B, Tsuji Atsushi

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Sugyo, Aya

× Sugyo, Aya

WEKO 474977

Sugyo, Aya

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Takuwa, Hiroyuki

× Takuwa, Hiroyuki

WEKO 474978

Takuwa, Hiroyuki

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Masamoto, Kazuto

× Masamoto, Kazuto

WEKO 474979

Masamoto, Kazuto

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Tomita, Yutaka

× Tomita, Yutaka

WEKO 474980

Tomita, Yutaka

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Suzuki, Norihiro

× Suzuki, Norihiro

WEKO 474981

Suzuki, Norihiro

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Imamura, Takeshi

× Imamura, Takeshi

WEKO 474982

Imamura, Takeshi

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Koizumi, Mitsuru

× Koizumi, Mitsuru

WEKO 474983

Koizumi, Mitsuru

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Saga, Tsuneo

× Saga, Tsuneo

WEKO 474984

Saga, Tsuneo

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須藤 仁美

× 須藤 仁美

WEKO 474985

en 須藤 仁美

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辻 厚至

× 辻 厚至

WEKO 474986

en 辻 厚至

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須尭 綾

× 須尭 綾

WEKO 474987

en 須尭 綾

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田桑 弘之

× 田桑 弘之

WEKO 474988

en 田桑 弘之

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正本 和人

× 正本 和人

WEKO 474989

en 正本 和人

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冨田 裕

× 冨田 裕

WEKO 474990

en 冨田 裕

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今村 剛

× 今村 剛

WEKO 474991

en 今村 剛

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小泉 満

× 小泉 満

WEKO 474992

en 小泉 満

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佐賀 恒夫

× 佐賀 恒夫

WEKO 474993

en 佐賀 恒夫

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抄録
内容記述タイプ Abstract
内容記述 Breast cancer is the most common cancer in women. Although advances in diagnostic imaging for early detection, surgical techniques and chemotherapy have improved overall survival, the prognosis of patients with metastatic breast cancer remains poor. Understanding cancer cell dynamics in the metastatic process is important to develop new therapeutic strategies. Experimental animal models and imaging would be powerful tools for understanding of the molecular events of multistep process of metastasis. In the present study, to develop a new cancer cell line that is applicable to bioluminescence and fluorescence imaging, we transfected the expression vector of a green fluorescent protein ZsGreen1 into a metastatic cell line 5a-D-Luc, which is a subclone of the MDA-MB-231 breast cancer cell line expressing luciferase, and established a new tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and ZsGreen1. The 5a-D-Luc-ZsGreen cells proliferate more rapidly and have a more invasive phenotype compared with 5a-D-Luc cells following intracardiac injection. Metastasis sites were easily detected in the whole body by bioluminescence imaging and in excised tissues by ex vivo fluorescence imaging. The fluorescence of 5a-D-Luc-ZsGreen cells was not lost after formalin fixation and decalcification. It enabled us to easily evaluate tumor spread and localization at the cellular level in microscopic analysis. The strong fluorescence of 5a-D-Luc-ZsGreen cells allowed for real-time imaging of circulating tumor cells in cerebral blood vessels of live animals immediately after intracardiac injection of cells using two-photon laser-scanning microscopy. These findings suggest that the 5a-D-Luc-ZsGreen cells would be a useful tool for research on mechanisms of metastatic process in animal models.
書誌情報 International journal of oncology

巻 48, 号 2, p. 525-532, 発行日 2015-12
出版者
出版者 D.A. Spandidos
ISSN
収録物識別子タイプ ISSN
収録物識別子 1019-6439
PubMed番号
識別子タイプ PMID
関連識別子 26691676
DOI
識別子タイプ DOI
関連識別子 10.3892/ijo.2015.3300
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