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Magnetic resonance imaging of mitochondrial dysfunction and metabolic activity, accompanied by overproduction of superoxide.
https://repo.qst.go.jp/records/47412
https://repo.qst.go.jp/records/474127fdf5bad-c7df-4e38-b0c7-ffba9be474cf
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-05-18 | |||||
| タイトル | ||||||
| タイトル | Magnetic resonance imaging of mitochondrial dysfunction and metabolic activity, accompanied by overproduction of superoxide. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Bakalova, Rumiana
× Bakalova, Rumiana× Georgieva, Ekaterina× Ivanova, Donika× Zhelev, Zhivko× Aoki, Ichio× Saga, Tsuneo× バカロバ ルミアナ× Ｇｅｏｒｇｉｅｖａ Ｅｋａｔｅｒｉｎａ× イヴァノヴァ ドニカ× Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ× 青木 伊知男× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study shows that mitochondria-penetrating nitroxide probe (mito-TEMPO) allows detection of superoxide and visualization of mitochondrial dysfunction in living cells due to the effect of T1 shortening in MRI. Mitochondrial dysfunction was induced by treatment of cells with rotenone and 2-methoxyestradiol (2-ME/Rot). The MRI measurements were performed on 7T MRI. The 2-ME/Rot-treated cells were characterized by overproduction of superoxide, which was confirmed by a conventional dihydroethidium-test. In the presence of mito-TEMPO, the intensity of MRI signal in 2-ME/Rot-treated cells was ~30-40% higher, in comparison with that in non-treated cells or culture media. In model (cell-free) systems, we observed that superoxide, but not hydrogen peroxide, increased the intensity of T1-weighted MRI signal of mito-TEMPO. Moreover, the superoxide restores the T1-weighted MRI contrast of mito-TEMPOH - a non-contrast (diamagnetic) analogue of mito-TEMPO. This was also confirmed by using EPR spectroscopy. The results demonstrate that superoxide radical is involved in the enhancement of T1-weighted MRI contrast in living cells - in the absence and presence of mito-TEMPO. This report gives a direction for discovering new opportunities for functional MRI - for detection of metabolic activity, accompanied by overproduction of superoxide, as well as by disturbance of the balance between superoxide and hydrogen peroxide - a very important approach to clarify the fine molecular mechanisms in the regulation of a lot of pathologies. The visualization of mitochondrial activity in real-time can be crucial to clarify the molecular mechanism of the functional MRI in its commonly accepted definition - as a method for detection of neurovascular coupling. | |||||
| 書誌情報 |
ACS chemical neuroscience 巻 6, 号 12, p. 1922-1929, 発行日 2015-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1948-7193 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 26367059 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1021/acschemneuro.5b00220 | |||||
