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High-throughput screening with nanoimprinting 3D culture for efficient drug development by mimicking the tumor environment
https://repo.qst.go.jp/records/47115
https://repo.qst.go.jp/records/4711582a0ed32-d178-4c0f-b262-5d6a24879b28
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-04-27 | |||||
| タイトル | ||||||
| タイトル | High-throughput screening with nanoimprinting 3D culture for efficient drug development by mimicking the tumor environment | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshii, Yukie
× Yoshii, Yukie× Furukawa, Takako× Waki, Atsuo× Okuyama, Hiroaki× Inoue, Masahiro× Itoh, Manabu× Zhang, Ming-Rong× Wakizaka, Hidekatsu× Sogawa, Chizuru× Kiyono, Yasushi× Yoshii, Hiroshi× Fujibayashi, Yasuhisa× Saga, Tsuneo× 吉井 幸恵× 古川 高子× 脇 厚生× 張 明栄× 脇坂 秀克× 曽川 千鶴× 吉井 裕× 藤林 康久× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Anti-cancer drug development typically utilizes high-throughput screening with two-dimensional (2D) cell culture. However, 2D culture induces cellular characteristics different from tumors in vivo, resulting in inefficient drug development. Here, we report an innovative high-throughput screening system using nanoimprinting 3D culture to simulate in vivo conditions, thereby facilitating efficient drug development. We demonstrated that cell line-based nanoimprinting 3D screening can more efficiently select drugs that effectively inhibit cancer growth in vivo as compared to 2D culture. Metabolic responses after treatment were assessed using positron emission tomography (PET) probes, and revealed similar characteristics between the 3D spheroids and in vivo tumors. Further, we developed an advanced method to adopt cancer cells from patient tumor tissues for high-throughput drug screening with nanoimprinting 3D culture, which we termed Cancer tissue-Originated Uniformed Spheroid Assay (COUSA). This system identified drugs that were effective in xenografts of the original patient tumors. Nanoimprinting 3D spheroids showed low permeability and formation of hypoxic regions inside, similar to in vivo tumors. Collectively, the nanoimprinting 3D culture provides easy-handling high-throughput drug screening system, which allows for efficient drug development by mimicking the tumor environment. The COUSA system could be a useful platform for drug development with patient cancer cells. | |||||
| 書誌情報 |
Biomaterials 巻 51, p. 278-289, 発行日 2015-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0142-9612 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | doi:10.1016/j.biomaterials.2015.02.008 | |||||
