WEKO3
アイテム
Novel characteristics of CtIP at damage-induced foci following the initiation of DNA end resection
https://repo.qst.go.jp/records/47073
https://repo.qst.go.jp/records/470734092d774-228e-4b36-adbc-670b55099c88
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-04-02 | |||||
| タイトル | ||||||
| タイトル | Novel characteristics of CtIP at damage-induced foci following the initiation of DNA end resection | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujisawa, Hiroshi
× Fujisawa, Hiroshi× Fujimori, Akira× Okayasu, Ryuichi× Uesaka, Mitsuru× Yajima, Hirohiko× 藤澤 寛× 藤森 亮× 岡安 隆一× 上坂 充× 矢島 浩彦 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Homologous recombination (HR) is a major repair pathway for DNA double strand breaks (DSBs), and end resection, which generates a 3’-single strand DNA tail at the DSB, is an early step in the process. Resection is initiated by the Mre11 nuclease together with CtIP. Here, we describe novel characteristics of CtIP at DSBs. At early times following exposure of human cells to ionizing radiation, CtIP localized to the DSB, became hyperphosphorylated and formed foci in an ATM-dependent manner. At later times, when the initiation of resection had occurred, CtIP foci persist but CtIP is maintained in a hypophosphorylated state, which is dependent on ATM and ATR. Exposure to cycloheximide revealed that CtIP turns over at DSB sites downstream of resection. Our findings provide strong evidence that CtIP is continuously recruited to DSBs downstream of both the initiation and extension step of resection, strongly suggesting that CtIP has functions in addition to promoting the initiation of resection during HR. | |||||
| 書誌情報 |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 巻 771, p. 36-44, 発行日 2014-12 |
|||||
| 出版者 | ||||||
| 出版者 | Elsevier Ltd. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0027-5107 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | doi:10.1016/j.mrfmmm.2014.12.001 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.sciencedirect.com/science/article/pii/S0027510714002073 | |||||
| 関連名称 | http://www.sciencedirect.com/science/article/pii/S0027510714002073 | |||||
