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Co-visualization of DNA damage and ion traversals in live mammalian cells using fluorescent nuclear track detector
https://repo.qst.go.jp/records/47053
https://repo.qst.go.jp/records/47053fa0cf1c1-4508-445d-884a-9db62324a9a2
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-03-25 | |||||
| タイトル | ||||||
| タイトル | Co-visualization of DNA damage and ion traversals in live mammalian cells using fluorescent nuclear track detector | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kodaira, Satoshi
× Kodaira, Satoshi× Konishi, Teruaki× Kobayashi, Alisa× Maeda, Takeshi× Ahbrizal, Farizal Tengku Ahmad Tengku× Yang, Gen× S, Akselrod Mark× Furusawa, Yoshiya× Uchihori, Yukio× 小平 聡× 小西 輝昭× 小林 亜利紗× 前田 武× 古澤 佳也× 内堀 幸夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The geometric locations of ion traversals in mammalian cells constitute important information in the study of heavy ion induced biological effect. Single ion traversal through a cellular nucleus produces complex and massive DNA damage at a nanometer level, leading to cell inactivation, mutations, and transformation. We present a novel approach that uses a fluorescent nuclear track detector (FNTD) for the simultaneous detection of the geometrical images of ion traversals and DNA damage in single cells using confocal microscopy. HT1080 or HT1080-53BP1-GFP cells were cultured on the surface of an FNTD and exposed to 5.1 MeV/n neon ions. The positions of the ion traversals were obtained as fluorescent images of FNDT. Localized DNA damage in cells was identified as fluorescent spots of γ-H2AX or 53BP1-GFP. These track images and images of damaged DNA were obtained in a short time using a confocal laser scanning microscope. The geometrical distribution of DNA damage indicated by fluorescent γ-H2AX spots in fixed cells or fluorescent 53BP1-GFP spots in living cells was found to correlate well with the distribution of the ion traversals. This method will be useful for evaluating the number of ion hits on individual cells, not only for micro-beam but also for random beam experiments. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 56, 号 2, p. 360-365, 発行日 2014-10 |
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| 出版者 | ||||||
| 出版者 | oxford university press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 25324538 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/jrr/rru091 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://jrr.oxfordjournals.org/content/early/2014/10/15/jrr.rru091.full | |||||
| 関連名称 | http://jrr.oxfordjournals.org/content/early/2014/10/15/jrr.rru091.full | |||||
