WEKO3
アイテム
Nonhomologous End-Joining Repair Plays a More Important Role than Homologous Recombination Repair in Defining Radiosensitivity after Exposure to High-LET Radiation
https://repo.qst.go.jp/records/46983
https://repo.qst.go.jp/records/4698390526f2e-a4a4-4de5-9963-0e5aadd60cf4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-12-11 | |||||
| タイトル | ||||||
| タイトル | Nonhomologous End-Joining Repair Plays a More Important Role than Homologous Recombination Repair in Defining Radiosensitivity after Exposure to High-LET Radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takahashi, Akihisa
× Takahashi, Akihisa× Makoto, Kubo× Hongyu, Ma× Akiko, Nakagawa× Yoshida, Yukari× Isono, Mayu× Kanai, Tatsuaki× Ohno, Tatsuya× Furusawa, Yoshiya× Funayama, Tomoo× Kobayashi, Yasuhiko× Nakano, Takashi× 高橋 昭久× 吉田 由香里× 磯野 真由× 金井 達明× 大野 達也× 古澤 佳也× 舟山 知夫× 小林 泰彦× 中野 隆史 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA double-strand breaks (DSBs) induced by ionizing radiation pose a major threat to cell survival. The cell can respond to the presence of DSBs through two major repair pathways: homologous recombination (HR) and non homologous end joining (NHEJ).Higher levels of cell death are induced by high-linear energy transfer (LET) radiation when compared to low-LET radiation, even at the same physical doses, due to less effective and efficient DNA repair. To clarify whether high-LET radiation inhibits all repair pathways or specifically one repair pathway, studies were designed to examine the effects of radiation with different LET values on DNA DSB repair and radiosensitivity. Embryonic fibroblasts bearing repair gene (NHEJ-related Lig4 and/or HR-related Rad54) knockouts (KO) were used and their responses were compared to wild-type cells. The cells were exposed to X rays, spread-out Bragg peak (SOBP) carbon ion beams as well as with carbon, iron, neon and argon ions. Cell survival was measured with colony-forming assays. The sensitization enhancement ratio (SER) values were calculated using the 10% survival dose of wild-type cells and repair-deficient cells. Cellular radiosensitivity was listed in descending order: double-KO cells . Lig4-KO cells . Rad54-KO cells . wild-type cells. Although Rad54-KO cells had an almost constant SER value, Lig4-KO cells showed a high-SER value when compared to Rad54-KO cells, even with increasing LET values. These results suggest that with carbon-ion therapy, targeting NHEJ repair yields higher radio sensitivity than targeting homologous recombination repair. | |||||
| 書誌情報 |
Radiation Research 巻 182, 号 3, p. 338-344, 発行日 2014-09 |
|||||
| 出版者 | ||||||
| 出版者 | Radiation Research Society / BioOne | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 25117625 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1667/RR13782.1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.bioone.org/doi/full/10.1667/RR13782.1 | |||||
| 関連名称 | http://www.bioone.org/doi/full/10.1667/RR13782.1 | |||||
