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A branching process model for the analysis of abortive colony size distributions in carbon ion-irradiated normal human fibroblasts
https://repo.qst.go.jp/records/46959
https://repo.qst.go.jp/records/4695990ffa97c-c235-4424-ada4-db39f091d5f4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-19 | |||||
| タイトル | ||||||
| タイトル | A branching process model for the analysis of abortive colony size distributions in carbon ion-irradiated normal human fibroblasts | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sakashita, Tetsuya
× Sakashita, Tetsuya× HAMADA, Nobuyuki× Kawaguchi, Isao× et.al× 川口 勇生 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A single cell can form a colony, and ionizing irradiation has long been known to reduce such a cellular clonogenic potential. Analysis of abortive colonies unable to continue to grow should provide important information on the reproductive cell death (RCD) following irradiation. Our previous analysis with a branching process model showed that the RCD in human fibroblasts can persist over 16 generations following irradiation with low linear energy transfer (LET) γ-rays. Here we further set out to evaluate the RCD persistency in abortive colonies arising from normal human fibroblasts exposed to high-LET carbon ions (18.3 MeV/u, 108 keV/µm). We found that the abortive colony size distribution determined by biological experiments follows the linear relationship on the log-log plot, and that the Monte Carlo simulation using the RCD probability estimated from such linear relationship well simulates the experimentally determined surviving fraction and the relative biological effectiveness (RBE). We identified the short-term phase and long-term phase for the persistent RCD, as was the case for γ-irradiation. Taken together, our results suggest that subsequent secondary or tertiary colony formation would be invaluable for understanding the long-lasting RCD. All together, our framework for analysis with a branching process model and a colony formation assay is applicable to determination of cellular responses to low- and high-LET radiation, and suggests that the long-lasting RCD is a pivotal determinant of the surviving fraction and the RBE. | |||||
| 書誌情報 |
Journal of radiation research 巻 55, 号 3, p. 423-431, 発行日 2014-05 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
