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False-positive TUNEL staining observed in SV40 based transgenic murine prostate cancer models.
https://repo.qst.go.jp/records/46939
https://repo.qst.go.jp/records/4693972727b61-a670-40af-b4f3-d1d3b8a3c8c6
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-17 | |||||
| タイトル | ||||||
| タイトル | False-positive TUNEL staining observed in SV40 based transgenic murine prostate cancer models. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
D, Lawrence M
× D, Lawrence M× J, Blyth B× J, Ormsby R× D, Tilley W× J, Sykes P× Ｂｌｙｔｈ Ｂｅｎｊａｍｉｎ |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The TRAMP (Transgenic Adenocarcinoma of the Mouse Prostate) and LADY (Probasin-large T antigen transgenic mouse) mice are widely used autochthonous models of prostate cancer. Both models utilise probasin promoters to direct androgen-regulated expression of oncogenic SV40 specifically to epithelial cells of the mouse prostate. The oncogenic processes and phenotypes which result mimic many features of human prostate cancer, making these transgenic mouse models useful experimental systems. The terminal deoxynucleotidyl transferase (Tdt)-mediated dUTP in situ nick end labelling (TUNEL) assay is a commonly used method for the detection of cells undergoing apoptosis. In this study, we demonstrate false-positive TUNEL staining in frozen prostate tissue from TRAMP and LADY mice, which was not observed in non-transgenic control animals and is not due to non-specific binding of labelled-dUTP substrate. The false-positive signal co-localised with large SV40 T-antigen expression. False-positive signal was apparent using multiple commercial TUNEL kits with different detection systems. These results caution against the use of the TUNEL assay for detection of apoptosis in frozen prostate tissue of large T-antigen based autochthonous transgenic models of prostate cancer. | |||||
| 書誌情報 |
Transgenic Research 巻 22, 号 5, p. 1037-1047, 発行日 2013-10 |
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| 出版者 | ||||||
| 出版者 | Kluwer Academic Publishers | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0962-8819 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 23423848 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s11248-013-9694-7 | |||||
