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Participation of Gap Junction Communication in Potentially Lethal Damage Repair and DNA Damage in Human Fibroblasts Exposed to Low- or High-LET Radiation
https://repo.qst.go.jp/records/46896
https://repo.qst.go.jp/records/46896f14d5b46-e63b-4baf-a109-aebc4bd4012b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-06 | |||||
| タイトル | ||||||
| タイトル | Participation of Gap Junction Communication in Potentially Lethal Damage Repair and DNA Damage in Human Fibroblasts Exposed to Low- or High-LET Radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Autsavapromporn, Narongchai
× Autsavapromporn, Narongchai× Suzuki, Masao× Plante, Ianik× Hua, Liu Cui× Uchihori, Yukio× K, Hei Tom× Azzam, Edouard× Murakami, Takeshi× アッサワプロンポーン ナロンチャイ× 鈴木 雅雄× 劉 翠華× 内堀 幸夫× 村上 健 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Existing research has not fully explained how different types of ionizing radiation (IR) modulate the responses of cell populations or tissues. In our previous work, we showed that gap junction intercellular communication (GJIC) mediates the propagation of stressful effects among irradiated cells exposed to high linear energy transfer (LET) radiations, in which almost every cells is traversed by an IR track. In the present study, we conducted an in-depth study of the role of GJIC in modulating the repair of potentially lethal damage (PLDR) and micronuclei formation in cells exposed to low- or high-LET IR. Confluent human fibroblasts were exposed in the presence or absence of a gap junction inhibitor to 200 kV X rays (LET 1.7 keV/µm), carbon ions (LET 76 keV/µm), silicon ions (LET 113 keV/µm) or iron ions (LET 400 keV/µm) that resulted in isosurvival levels. The fibroblasts were incubated for various times at 37oC. As expected, high-LET IR were more effective than were low-LET X rays at killing cells and damaging DNA shortly after irradiation. However, when cells were held in a confluent state for several hours, PLDR associated with a reduction in DNA damage, occurred only in cells exposed to X rays. Interestingly, inhibition of GJIC eliminated the enhancement of toxic effects, which resulted in an increase of cell survival and reduction in the level of micronucleus formation in cells exposed to high, but not in those exposed to low-LET IR. The experiment shows that gap- junction communication plays an important role in the propagation of stressful effects among irradiated cells exposed to high-LET IR while GJIC has only a minimal effect on PLDR and DNA damage following low-LET irradiation. Together, our results show that PLDR and induction of DNA damage clearly depend on gap-junction communication and radiation quality. | |||||
| 書誌情報 |
Genetic Toxicology and Environmental Mutagenesis : A Section of Mutation Research 巻 756, 号 1/2, p. 78-85, 発行日 2013-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1383-5718 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.1016/j.mrgentox.2013.07.001 | |||||
| 関連名称 | http://dx.doi.org/10.1016/j.mrgentox.2013.07.001 | |||||
