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Differential effects of p53 on bystander phenotypes induced by gamma ray and high LET heavy ion radiation
https://repo.qst.go.jp/records/46801
https://repo.qst.go.jp/records/4680115299d67-0728-4cb3-b869-7f274de832fc
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-07-30 | |||||
| タイトル | ||||||
| タイトル | Differential effects of p53 on bystander phenotypes induced by gamma ray and high LET heavy ion radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
He, Mingyuan
× He, Mingyuan× Dong, Chen× Konishi, Teruaki× Tu, Wenzhi× Liu, Weili× Shiomi, Naoko× Kobayashi, Alisa× Uchihori, Yukio× Furusawa, Yoshiya× K., Hei Tom× Dang, Bingrong× Shao, Chunlin× 小西 輝昭× 塩見 尚子× 小林 亜利紗× 内堀 幸夫× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | High LET particle irradiation has several potential advantages over γ -rays such as p53-independent response. The purpose of this work is to disclose the effect of p53 on the bystander effect induced by different LET irradiations and underlying mechanism. Lymphocyte cells of TK6 (wild type p53) and HMy2.CIR (mutated p53) were exposed to either low or high LET irradiation, then their mitochondrial dysfunction and ROS generation were detected. The micronuclei (MN) induction in HL-7702 hepatocytes co-cultured with irradiated lymphocytes was also measured. It was found that the mitochondrial dysfunction, p66Shc activation, and intracellular ROS were enhanced in TK6 but not in HMy2.CIR cells after γ -ray irradiation, but all of them were increased in both cell lines after carbon and iron irradiation. Consistently, the bystander effect of MN formation in HL-7702 cells was only triggered by γ -irradiated TK6 cells but not by γ -irradiated HMy2.CIR cells. But this bystander effect was induced by both lymphocyte cell lines after heavy ion irradiation. PFT-μ, an inhibitor of p53, only partly inhibited ROS generation and bystander effect induced by 30 keV/μm carbon-irradiated TK6 cells but failed to suppress the bystander effect induced by the TK6 cells irradiated with either 70 keV/μm carbon or 180 keV/μm iron. The mitochondrial inhibitors of rotenone and oligomycin eliminated heavy ion induced ROS generation in TK6 and HMy2.CIR cells and hence diminished the bystander effect on HL-7702 cells. These results clearly demonstrate that the bystander effect is p53-dependent for low LET irradiation, but it is p53-independent for high LET irradiation which may be because of p53-independent ROS generation due to mitochondrial dysfunction. | |||||
| 書誌情報 |
Life Sciences in Space Research 巻 1, p. 53-59, 発行日 2014-04 |
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| 出版者 | ||||||
| 出版者 | Elsevier Ltd. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2214-5524 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.lssr.2014.02.003 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.1016/j.lssr.2014.02.003 | |||||
| 関連名称 | http://dx.doi.org/10.1016/j.lssr.2014.02.003 | |||||
