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Most hydrogen peroxide-induced histone H2AX phosphorylation is mediated by ATR and is not dependent on DNA double-strand breaks
https://repo.qst.go.jp/records/46793
https://repo.qst.go.jp/records/46793e47443c6-cbb4-406c-bc65-115bb5a9a48d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-07-29 | |||||
| タイトル | ||||||
| タイトル | Most hydrogen peroxide-induced histone H2AX phosphorylation is mediated by ATR and is not dependent on DNA double-strand breaks | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
勝部, 孝則
× 勝部, 孝則× 森, 雅彦× 辻, 秀雄× 塩見, 忠博× 王, 冰× 根井, 充× 小野田, 眞× et.al× 勝部 孝則× 森 雅彦× 辻 秀雄× 塩見 忠博× 王 冰× 根井 充× 小野田 眞 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The nuclear foci of phosphorylated histone H2AX (cH2AX) are frequently used as a marker for DNA double-strand breaks (DSBs) following ionizing radi- ation (IR). However, recent studies reported that cH2AX foci do not necessarily correlate with DSBs under other conditions. We showed that cH2AX foci induced by oxidative stress in hydrogen peroxide (H2O2)-treated cells displayed several different features from those induced by IR. The magnitude of cH2AX induction was heterogeneous among H2O2-treated cells. Some cells expressed small discrete cH2AX foci, whereas others expressed a gross cH2AX signal that was distributed throughout the nucleus. Oxidative stress-induced cH2AX was eliminated in DSB repair- deficient mutant cells as efficiently as in wild-type cells and was not necessarily accompanied by phosphorylated ataxia telangiectasia mutated (ATM) or 53BP1 foci. Analyses using specific inhibitors showed that ATM- and Rad3-related (ATR), rather than ATM, was the prominent kinase mediating the oxidative stress re- sponse. These results suggest that a major fraction of cH2AX induced by oxidative stress is not associated with DSBs. Single-stranded DNA arisen from stalled replication forks can cause the ATR-mediated induction of cH2AX. However, oxidative stress appeared to induce cH2AX in both S- and non-S-phase cells. These results suggest that there may be another path- way leading to the ATR-mediated induction of cH2AX in non-S-phase cells without DSBs. | |||||
| 書誌情報 |
Journal of Biochemistry 巻 156, 号 2, p. 85-95, 発行日 2014-04 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021-924X | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 24682951 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | doi:10.1093/jb/mvu021 | |||||
