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Imaging of superoxide generation in the dopaminergic area of the brain in Parkinson's disease, using mito-TEMPO
https://repo.qst.go.jp/records/46783
https://repo.qst.go.jp/records/46783aa94582e-fc3b-4dec-a942-be4535031994
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-07-22 | |||||
| タイトル | ||||||
| タイトル | Imaging of superoxide generation in the dopaminergic area of the brain in Parkinson's disease, using mito-TEMPO | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Zhelev, Zhivko
× Zhelev, Zhivko× Bakalova-Zheleva, Rumiana× Aoki, Ichio× Lazarova, Dessislava× Saga, Tsuneo× バカロバ ルミアナ× 青木 伊知男× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We report a new methodology for direct visualization of superoxide production in the dopaminergic area of the brain in Parkinson's disease, based on the redox cycle of mito-TEMPO, a blood-brain barrier-, cell-, and mitochondria-penetrating nitroxide derivative with superoxide scavenging properties and T1 magnetic resonance imaging (MRI) contrast. The experiments were conducted on healthy and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. In healthy mice, the nitroxide-enhanced MRI signal was weak and short-lived (half-life ∼ 40 s; duration ∼ 80 s). The profile of the histograms indicated a high reducing activity of normal brain tissues against mito-TEMPO. In MPTP-treated mice, the nitroxide-enhanced MRI signal was strong and long-lived (half-life > 20 min; duration > 20 min), especially in the dopaminergic area of the brain. The histograms indicated a high oxidative activity in dopaminergic tissues of MPTP-treated mice. The results show directly, on intact mammals, that superoxide is a major inducer and/or mediator of neurodegenerative damage in Parkinson's disease. The high oxidative status of brain tissue in Parkinson's disease was also confirmed on isolated tissue specimens, using total reducing capacity assay and ROS/RNS assay. | |||||
| 書誌情報 |
ACS Chemical Neuroscience 巻 4, 号 11, p. 1439-1445, 発行日 2013-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1948-7193 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | doi.org/10.1021/cn400159h | |||||
