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Expression and Functional Analysis of the CorA-MRS2-ALR-Type Magnesium Transporter Family in Rice
https://repo.qst.go.jp/records/46743
https://repo.qst.go.jp/records/467438eff41dc-4fea-4fc6-b318-dcb0d70e79fd
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-18 | |||||
| タイトル | ||||||
| タイトル | Expression and Functional Analysis of the CorA-MRS2-ALR-Type Magnesium Transporter Family in Rice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Saito, Takayuki
× Saito, Takayuki× Kobayashi, Natsuko× Tanoi, Keitaro× Iwata, Naoko× Suzuki, Hisashi× Iwata, Ren× Nakanishi, Tomoko× 斉藤 貴之× 田野井 慶太朗× 鈴木 寿× 岩田 錬× 中西 友子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Maintenance of an appropriate magnesium ion (Mg2+) concentration is essential for plant growth. In Arabidopsis thaliana, the CorA-MRS2-ALR-type proteins, named MRS2/MGT family proteins, are reportedly localized in various membranes and they function in Mg transport. However, knowledge of this family in other plant species is extremely limited. Furthermore, differential diversification among dicot and monocot plants suggested by phylogenetic analysis indicates that the role of the Arabidopsis MRS2/MGT family proteins is not the same in monocot plants. For a further understanding of this family in higher plants, functional analysis and gene expression profiling of rice MRS2/MGT family members were performed. A phylogenetic tree based on the isolated mRNA sequences of nine members of the OsMRS2 family confirmed that the MRS2/MGT family consists of five clades (A–E). A complementation assay in the yeast CM66 strain showed that four of the nine members possessed the Mg2+ transport ability. Transient green fluorescent protein (GFP) expression in the isolated rice protoplast indicated that OsMRS2-5 and OsMRS2-6, belonging to clades D and A, respectively, localized in the chloroplast. Expression levels of these genes were low in the unexpanded yellow–green leaf, but increased considerably with leaf maturation. In addition, diurnal oscillation of expression was observed, particularly in OsMRS2-6 expression in the expanded leaf blade. We conclude that OsMRS2 family members function as Mg transporters and suggest that the genes belonging to clade A encode the chloroplast-localized Mg2+ transporter in plants. | |||||
| 書誌情報 |
Plant Cell Physiology 巻 54, 号 10, p. 1673-1683, 発行日 2013-08 |
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| 出版者 | ||||||
| 出版者 | OXFORD JOURNALS | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0032-0781 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | doi:10.1093/pcp/pct112 | |||||
