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Novel system using microliter order sample volume for measuring arterial radioactivity concentrations in whole blood and plasma for mouse PET dynamic study
https://repo.qst.go.jp/records/46616
https://repo.qst.go.jp/records/46616dd40990d-46b0-4072-b328-2eeba1537031
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-10-30 | |||||
| タイトル | ||||||
| タイトル | Novel system using microliter order sample volume for measuring arterial radioactivity concentrations in whole blood and plasma for mouse PET dynamic study | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kimura, Yuichi
× Kimura, Yuichi× Seki, Chie× Hashizume, Nobuya× Yamada, Takashi× Wakizaka, Hidekatsu× Nishimoto, Takahiro× Hatano, Kentaro× Kitamura, Keishi× Toyama, Hiroshi× Kanno, Iwao× 木村 裕一× 関 千江× 脇坂 秀克× 北村 圭司× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study aimed to develop a new system, named CD-Well, for mouse PET dynamic study. CD-Well allows the determination of time-activity curves(TACs) for arterial whole blood and plasma using 2-3 muL of blood per sample;the minute sample size is ideal for studies in small animals. The system has the following merits: (1)measures volume and radioactivity of whole blood and plasma separately; (2) allows measurements at 10 s intervals to capture initial rapid changes in the TAC; and (3) is compact and easy to handle, minimizes blood loss from sampling, and delay and dispersion of the TAC. CD-Well has 36 U-shaped channels. A drop of blood is sampled into the opening of the channel and stored there. After serial sampling is completed, CD-Well is centrifuged and scanned using a flatbed scanner to define the regions of plasma and blood cells. The length measured is converted to volume because the channels have a precise and uniform cross section. Then, CD-Well is exposed to an imaging plate to measure radioactivity. Finally, radioactivity concentrations are computed. We evaluated the performance of CD-Well in in vitro measurement and in vivo 18F-fluorodeoxyglucose and [11C]2-carbomethoxy-3beta-(4-fluorophenyl) tropane studies. In in vitro evaluation, per cent differences (mean+-SE) from manual measurement were 4.4+-3.6% for whole blood and 4.0+-3.5% for plasma across the typical range of radioactivity measured in mouse dynamic study. In in vivo studies, reasonable TACs were obtained. The peaks were captured well, and the time courses coincided well with the TAC derived from PET imaging of the heart chamber. The total blood loss was less than 200 muL, which had no physiological effect on the mice. CD-Well demonstrates satisfactory performance, and is useful for mouse PETdynamic study. |
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| 書誌情報 |
Physics in Medicine and Biology 巻 58, 号 22, p. 7889-7903, 発行日 2013-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0031-9155 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1088/0031-9155/58/22/7889 | |||||
