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In vivo visualization of the lymphatic vessels in pFLT4-EGFP transgenic medaka.
https://repo.qst.go.jp/records/46600
https://repo.qst.go.jp/records/4660049679207-7256-471f-bfaa-bc74ba29f01a
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-10-08 | |||||
| タイトル | ||||||
| タイトル | In vivo visualization of the lymphatic vessels in pFLT4-EGFP transgenic medaka. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Deguchi, Tomonori
× Deguchi, Tomonori× Fujimori, Kazuhiro× Kawasaki, Takashi× Maruyama, Kouichi× Yuba, Shunsuke× 丸山 耕一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | FMS-like tyrosine kinase 4 (FLT4) is a marker for lymphatic vessels and some high endothelial venules in human adult tissues. We generated a transgenic medaka fish in which the lymphatic vessels and some blood vessels are visible in vivo by transferring the promoter of medaka flt4 driving the expression of enhanced green fluorescent protein (EGFP), using a see-through medaka line. To do this, we identified and cloned medaka flt4, and generated a construct in which the promoter was the 4-kb region upstream of the translation initiation site. The fluorescent signal of EGFP could be observed with little background, and the expression pattern correlated well with that of flt4 determined by whole-mount RNA in situ hybridization. Because a see-through medaka line is transparent until adult, the model is useful for visualizing the lymphatic vessels not only in embryo and fry, but also in adult. This model will be a useful tool for analyzing lymphatic development. | |||||
| 書誌情報 |
Genesis 巻 50, 号 8, p. 625-634, 発行日 2012-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1526-954X | |||||
