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Monitoring Neuroprotective Effects Using Positron Emission Tomography With [11C]ITMM, a Radiotracer for Metabotropic Glutamate 1 Receptor
https://repo.qst.go.jp/records/46591
https://repo.qst.go.jp/records/46591a00061bc-258a-4f2c-a129-0d0142cb80d3
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-06 | |||||
| タイトル | ||||||
| タイトル | Monitoring Neuroprotective Effects Using Positron Emission Tomography With [11C]ITMM, a Radiotracer for Metabotropic Glutamate 1 Receptor | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yui, Joji
× Yui, Joji× Xie, Lin× Fujinaga, Masayuki× Yamasaki, Tomoteru× Hatori, Akiko× Kumata, Katsushi× Nengaki, Nobuki× Zhang, Ming-Rong× 由井 譲二× 謝 琳× 藤永 雅之× 山崎 友照× 羽鳥 晶子× 熊田 勝志× 念垣 信樹× 張 明栄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background and Purpose—Recent pharmacological evidence shows that antagonists for the metabotropic glutamate 1 (mGlu1) receptor exhibit neuroprotective effects in an ischemic brain. The aim of this study was to visualize the mGlu1 receptor and to monitor neuroprotective effects in a rat model of mild focal ischemia using positron emission tomography (PET) with N-[4-[6-(isopropylamino)pyrimidin-4-yl]-1,3-thiazol-2-yl]-4-[11C]methoxy-N-methylbenzamide ([11C]ITMM), a radiotracer for mGlu1. Methods—Rats were subjected to a 30-minute transient right middle cerebral artery occlusion. Saline or minocycline, a neuroprotective agent, was intravenously injected immediately after surgery and then daily during the subsequent 7 days. PET imaging with [11C]ITMM was performed on the rats on days 1 to 7 after ischemia. In vitro autoradiography and histopathologic staining were conducted to confirm the results of in vivo PET. Results—PET with [11C]ITMM demonstrated a gradual decrease of radioactivity in the ipsilateral sides of the ischemic brains. The radioactivity uptake ratio between the ipsilateral and contralateral sides also decreased with time. Minocycline treatment slowed down the decrease in the radioactivity level in the ipsilateral sides. Pretreatment with JNJ16259685, an mGlu1-selective ligand, significantly reduced brain radioactivity, confirming that the uptake of [11C]ITMM primarily reflects mGlu1 levels in the brain regions, including the ischemic area. In vitro autoradiography and histopathology confirmed the changes in mGlu1 levels in the brains. Conclusions—[11C]ITMM-PET may be a useful technique for characterizing the change in mGlu1 level during the occurrence and progression of neuronal damage and for evaluating the neuroprotective effects of drugs after ischemia. |
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| 書誌情報 |
Stroke 巻 44, 号 9, p. 2567-2572, 発行日 2013-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0039-2499 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1161/strokeaha.113.001178 | |||||
